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4yph

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==Crystal Structure of MutY bound to its anti-substrate with the disulfide cross-linker reduced==
==Crystal Structure of MutY bound to its anti-substrate with the disulfide cross-linker reduced==
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<StructureSection load='4yph' size='340' side='right' caption='[[4yph]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
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<StructureSection load='4yph' size='340' side='right'caption='[[4yph]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4yph]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YPH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4yph]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YPH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yoq|4yoq]], [[4ypr|4ypr]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yph OCA], [https://pdbe.org/4yph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yph RCSB], [https://www.ebi.ac.uk/pdbsum/4yph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yph ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yph OCA], [http://pdbe.org/4yph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yph RCSB], [http://www.ebi.ac.uk/pdbsum/4yph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yph ProSAT]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTY_GEOSE MUTY_GEOSE] Base excision repair (BER) glycosylase that initiates repair of A:oxoG to C:G by removing the inappropriately paired adenine base from the DNA backbone, generating an abasic site product (PubMed:25995449) (PubMed:14961129). 8-oxoguanine (oxoG) is a genotoxic DNA lesion resulting from oxidation of guanine; this residue is misread by replicative DNA polymerases, that insert adenine instead of cytosine opposite the oxidized damaged base. Shows a powerful dicrimination of A versus C, since it does not cleave cytosine in oxoG:C pairs (PubMed:25995449). May also be able to remove adenine from A:G mispairs, although this activity may not be physiologically relevant (PubMed:14961129).<ref>PMID:25995449</ref> <ref>PMID:14961129</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
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*[[DNA glycosylase|DNA glycosylase]]
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lee, S]]
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Verdine, G L]]
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[[Category: Large Structures]]
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[[Category: Wang, L]]
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[[Category: Synthetic construct]]
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[[Category: 8-oxoguanine]]
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[[Category: Lee S]]
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[[Category: Anti-substrate]]
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[[Category: Verdine GL]]
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[[Category: Base-excision repair]]
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[[Category: Wang L]]
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[[Category: Hydrolase-dna complex]]
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Current revision

Crystal Structure of MutY bound to its anti-substrate with the disulfide cross-linker reduced

PDB ID 4yph

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