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| | <StructureSection load='4z20' size='340' side='right'caption='[[4z20]], [[Resolution|resolution]] 3.20Å' scene=''> | | <StructureSection load='4z20' size='340' side='right'caption='[[4z20]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z20]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sormk Sormk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z20 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z20]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sordaria_macrospora_k-hell Sordaria macrospora k-hell] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z20 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z1z|4z1z]], [[4yhx|4yhx]], [[4yis|4yis]], [[4yit|4yit]], [[5esp|5esp]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMAC_12671 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=771870 SORMK])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z20 OCA], [https://pdbe.org/4z20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z20 RCSB], [https://www.ebi.ac.uk/pdbsum/4z20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z20 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z20 OCA], [http://pdbe.org/4z20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z20 RCSB], [http://www.ebi.ac.uk/pdbsum/4z20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z20 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F7WD42_SORMK F7WD42_SORMK] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sormk]] | + | [[Category: Sordaria macrospora k-hell]] |
| - | [[Category: Hallinan, J P]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Stoddard, B L]] | + | [[Category: Hallinan JP]] |
| - | [[Category: Homing endonuclease]] | + | [[Category: Stoddard BL]] |
| - | [[Category: Hydrolase-dna complex]]
| + | |
| - | [[Category: Laglidadg]]
| + | |
| - | [[Category: Meganuclease]]
| + | |
| Structural highlights
Function
F7WD42_SORMK
Publication Abstract from PubMed
LAGLIDADG meganucleases are DNA cleaving enzymes used for genome engineering. While their cleavage specificity can be altered using several protein engineering and selection strategies, their overall targetability is limited by highly specific indirect recognition of the central four base pairs within their recognition sites. In order to examine the physical basis of indirect sequence recognition and to expand the number of such nucleases available for genome engineering, we have determined the target sites, DNA-bound structures, and central four cleavage fidelities of nine related enzymes. Subsequent crystallographic analyses of a meganuclease bound to two noncleavable target sites, each containing a single inactivating base pair substitution at its center, indicates that a localized slip of the mutated base pair causes a small change in the DNA backbone conformation that results in a loss of metal occupancy at one binding site, eliminating cleavage activity.
Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.,Lambert AR, Hallinan JP, Shen BW, Chik JK, Bolduc JM, Kulshina N, Robins LI, Kaiser BK, Jarjour J, Havens K, Scharenberg AM, Stoddard BL Structure. 2016 Apr 27. pii: S0969-2126(16)30035-1. doi:, 10.1016/j.str.2016.03.024. PMID:27133026[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lambert AR, Hallinan JP, Shen BW, Chik JK, Bolduc JM, Kulshina N, Robins LI, Kaiser BK, Jarjour J, Havens K, Scharenberg AM, Stoddard BL. Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity. Structure. 2016 Apr 27. pii: S0969-2126(16)30035-1. doi:, 10.1016/j.str.2016.03.024. PMID:27133026 doi:http://dx.doi.org/10.1016/j.str.2016.03.024
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