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| | <StructureSection load='4zyr' size='340' side='right'caption='[[4zyr]], [[Resolution|resolution]] 3.31Å' scene=''> | | <StructureSection load='4zyr' size='340' side='right'caption='[[4zyr]], [[Resolution|resolution]] 3.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zyr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zyr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9PG:4-NITROPHENYL+ALPHA-D-GALACTOPYRANOSIDE'>9PG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.312Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pv7|1pv7]], [[2v8n|2v8n]], [[2y5y|2y5y]], [[4oaa|4oaa]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9PG:4-NITROPHENYL+ALPHA-D-GALACTOPYRANOSIDE'>9PG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacY, b0343, JW0334 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyr OCA], [https://pdbe.org/4zyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyr RCSB], [https://www.ebi.ac.uk/pdbsum/4zyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyr OCA], [http://pdbe.org/4zyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyr RCSB], [http://www.ebi.ac.uk/pdbsum/4zyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). | + | [https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Finer-Moore, J S]] | + | [[Category: Finer-Moore JS]] |
| - | [[Category: Kaback, H R]] | + | [[Category: Kaback HR]] |
| - | [[Category: Kumar, H]] | + | [[Category: Kumar H]] |
| - | [[Category: Stroud, R M]] | + | [[Category: Stroud RM]] |
| - | [[Category: Alpha-helical]]
| + | |
| - | [[Category: Membrane protein]]
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| - | [[Category: Transport protein]]
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| - | [[Category: Transporter]]
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| Structural highlights
Function
LACY_ECOLI Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
Publication Abstract from PubMed
The X-ray crystal structure of a conformationally constrained mutant of the Escherichia coli lactose permease (the LacY double-Trp mutant Gly-46-->Trp/Gly-262-->Trp) with bound p-nitrophenyl-alpha-d-galactopyranoside (alpha-NPG), a high-affinity lactose analog, is described. With the exception of Glu-126 (helix IV), side chains Trp-151 (helix V), Glu-269 (helix VIII), Arg-144 (helix V), His-322 (helix X), and Asn-272 (helix VIII) interact directly with the galactopyranosyl ring of alpha-NPG to provide specificity, as indicated by biochemical studies and shown directly by X-ray crystallography. In contrast, Phe-20, Met-23, and Phe-27 (helix I) are within van der Waals distance of the benzyl moiety of the analog and thereby increase binding affinity nonspecifically. Thus, the specificity of LacY for sugar is determined solely by side-chain interactions with the galactopyranosyl ring, whereas affinity is increased by nonspecific hydrophobic interactions with the anomeric substituent.
Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site.,Kumar H, Finer-Moore JS, Kaback HR, Stroud RM Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi:, 10.1073/pnas.1509854112. Epub 2015 Jul 8. PMID:26157133[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kumar H, Finer-Moore JS, Kaback HR, Stroud RM. Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi:, 10.1073/pnas.1509854112. Epub 2015 Jul 8. PMID:26157133 doi:http://dx.doi.org/10.1073/pnas.1509854112
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