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| | <StructureSection load='5cy5' size='340' side='right'caption='[[5cy5]], [[Resolution|resolution]] 3.40Å' scene=''> | | <StructureSection load='5cy5' size='340' side='right'caption='[[5cy5]], [[Resolution|resolution]] 3.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cy5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermoplasma_acidophila"_(sic)_darland_et_al._1970 "thermoplasma acidophila" (sic) darland et al. 1970] and [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CY5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5CY5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cy5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] and [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CY5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CY5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5cy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cy5 OCA], [http://pdbe.org/5cy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cy5 RCSB], [http://www.ebi.ac.uk/pdbsum/5cy5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cy5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cy5 OCA], [https://pdbe.org/5cy5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cy5 RCSB], [https://www.ebi.ac.uk/pdbsum/5cy5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cy5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pyrococcus shinkaii]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boyken, S]] | + | [[Category: Pyrococcus horikoshii]] |
| - | [[Category: Cannon, K A]] | + | [[Category: Thermoplasma acidophilum]] |
| - | [[Category: Cascio, D]] | + | [[Category: Boyken S]] |
| - | [[Category: King, N]] | + | [[Category: Cannon KA]] |
| - | [[Category: Park, R]] | + | [[Category: Cascio D]] |
| - | [[Category: Yeates, T O]] | + | [[Category: King N]] |
| - | [[Category: Biomaterial]] | + | [[Category: Park R]] |
| - | [[Category: Bionanotechnology]] | + | [[Category: Yeates TO]] |
| - | [[Category: Computational design]]
| + | |
| - | [[Category: De novo protein]]
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| - | [[Category: Nanomaterial]]
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| - | [[Category: Rosetta]]
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| - | [[Category: Self-assembly]]
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| - | [[Category: Solubility]]
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| - | [[Category: Symmetry]]
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| - | [[Category: Tetrahedral]]
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| Structural highlights
Publication Abstract from PubMed
In recent years, new protein engineering methods have produced more than a dozen symmetric, self-assembling protein cages whose structures have been validated to match their design models with near-atomic accuracy. However, many protein cage designs that are tested in the lab do not form the desired assembly, and improving the success rate of design has been a point of recent emphasis. Here we present two protein structures solved by X-ray crystallography of designed protein oligomers that form two-component cages with tetrahedral symmetry. To improve on the past tendency toward poorly soluble protein, we used a computational protocol that favors the formation of hydrogen-bonding networks over exclusively hydrophobic interactions to stabilize the designed protein-protein interfaces. Preliminary characterization showed highly soluble expression, and solution studies indicated successful cage formation by both designed proteins. For one of the designs, a crystal structure confirmed at high resolution that the intended tetrahedral cage was formed, though several flipped amino acid side chain rotamers resulted in an interface that deviates from the precise hydrogen-bonding pattern that was intended. A structure of the other designed cage showed that, under the conditions where crystals were obtained, a noncage structure was formed wherein a porous 3D protein network in space group I21 3 is generated by an off-target twofold homomeric interface. These results illustrate some of the ongoing challenges of developing computational methods for polar interface design, and add two potentially valuable new entries to the growing list of engineered protein materials for downstream applications.
Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering.,Cannon KA, Park RU, Boyken SE, Nattermann U, Yi S, Baker D, King NP, Yeates TO Protein Sci. 2020 Apr;29(4):919-929. doi: 10.1002/pro.3802. Epub 2019 Dec 26. PMID:31840320[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cannon KA, Park RU, Boyken SE, Nattermann U, Yi S, Baker D, King NP, Yeates TO. Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering. Protein Sci. 2020 Apr;29(4):919-929. doi: 10.1002/pro.3802. Epub 2019 Dec 26. PMID:31840320 doi:http://dx.doi.org/10.1002/pro.3802
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