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| | <StructureSection load='5kmx' size='340' side='right'caption='[[5kmx]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='5kmx' size='340' side='right'caption='[[5kmx]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kmx]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryci Tryci]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KMX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KMX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kmx]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense_IL3000 Trypanosoma congolense IL3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KMX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.452Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TCIL3000_10_9440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1068625 TRYCI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kmx OCA], [https://pdbe.org/5kmx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kmx RCSB], [https://www.ebi.ac.uk/pdbsum/5kmx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kmx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kmx OCA], [http://pdbe.org/5kmx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kmx RCSB], [http://www.ebi.ac.uk/pdbsum/5kmx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kmx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0UXP9_TRYCI G0UXP9_TRYCI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tryci]] | + | [[Category: Trypanosoma congolense IL3000]] |
| - | [[Category: Boulanger, M J]] | + | [[Category: Boulanger MJ]] |
| - | [[Category: Parker, M L]] | + | [[Category: Parker ML]] |
| - | [[Category: Ramaswamy, R]] | + | [[Category: Ramaswamy R]] |
| - | [[Category: Bi-lobed architecture]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Putative sensor]]
| + | |
| - | [[Category: Surface protein]]
| + | |
| Structural highlights
Function
G0UXP9_TRYCI
Publication Abstract from PubMed
African trypanosomiasis, caused by parasites of the genus Trypanosoma, is a complex of devastating vector-borne diseases of humans and livestock in sub-Saharan Africa. Central to the pathogenesis of African trypanosomes is their transmission by the arthropod vector, Glossina spp. (tsetse fly). Intriguingly, the efficiency of parasite transmission through the vector is reduced following depletion of Trypanosoma brucei Procyclic-Specific Surface Antigen-2 (TbPSSA-2). To investigate the underlying molecular mechanism of TbPSSA-2, we determined the crystal structures of its ectodomain and that of its homolog T. congolense Insect Stage Antigen (TcISA) to resolutions of 1.65 A and 2.45 A, respectively using single wavelength anomalous dispersion. Both proteins adopt a novel bilobed architecture with the individual lobes displaying rotational flexibility around the central tether that suggest a potential mechanism for coordinating a binding partner. In support of this hypothesis, electron density consistent with a bound peptide was observed in the inter-lob cleft of a TcISA monomer. These first reported structures of insect stage transmembrane proteins expressed by African trypanosomes provide potentially valuable insight into the interface between parasite and tsetse vector.
Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA.,Ramaswamy R, Goomeshi Nobary S, Eyford BA, Pearson TW, Boulanger MJ Protein Sci. 2016 Dec;25(12):2297-2302. doi: 10.1002/pro.3053. Epub 2016 Oct 13. PMID:27671214[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramaswamy R, Goomeshi Nobary S, Eyford BA, Pearson TW, Boulanger MJ. Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA. Protein Sci. 2016 Dec;25(12):2297-2302. doi: 10.1002/pro.3053. Epub 2016 Oct 13. PMID:27671214 doi:http://dx.doi.org/10.1002/pro.3053
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