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1n47

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[[Image:1n47.gif|left|200px]]
[[Image:1n47.gif|left|200px]]
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{{Structure
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|PDB= 1n47 |SIZE=350|CAPTION= <scene name='initialview01'>1n47</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1n47", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TNR:O-(2-ACETAMIDO-2-DEOXY-ALPHA-D-GALACTOPYRANOSYL)-L-SERINE'>TNR</scene>
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{{STRUCTURE_1n47| PDB=1n47 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n47 OCA], [http://www.ebi.ac.uk/pdbsum/1n47 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n47 RCSB]</span>
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'''Isolectin B4 from Vicia villosa in complex with the Tn antigen'''
'''Isolectin B4 from Vicia villosa in complex with the Tn antigen'''
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==About this Structure==
==About this Structure==
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1N47 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_villosa Vicia villosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N47 OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N47 OCA].
==Reference==
==Reference==
The crystal structure of a plant lectin in complex with the Tn antigen., Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM, FEBS Lett. 2003 Feb 11;536(1-3):106-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12586347 12586347]
The crystal structure of a plant lectin in complex with the Tn antigen., Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM, FEBS Lett. 2003 Feb 11;536(1-3):106-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12586347 12586347]
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[[Category: Protein complex]]
 
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[[Category: Vicia villosa]]
 
[[Category: Alzari, P M.]]
[[Category: Alzari, P M.]]
[[Category: Babino, A.]]
[[Category: Babino, A.]]
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[[Category: Rojas, A.]]
[[Category: Rojas, A.]]
[[Category: Tello, D.]]
[[Category: Tello, D.]]
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[[Category: cancer antigen]]
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[[Category: Cancer antigen]]
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[[Category: carbohydrate recognition]]
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[[Category: Carbohydrate recognition]]
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[[Category: glycoprotein tn-binding protein]]
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[[Category: Glycoprotein tn-binding protein]]
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[[Category: vicia villosa lectin]]
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[[Category: Vicia villosa lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:04:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:07 2008''
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Revision as of 23:04, 2 May 2008

Template:STRUCTURE 1n47

Isolectin B4 from Vicia villosa in complex with the Tn antigen


Overview

The structure of the tetrameric Vicia villosa isolectin B4 (VVLB4) in complex with a cancer antigen, the Tn glycopeptide (GalNAc-O-Ser), was determined at 2.7 A resolution. The N-acetylgalactoside moiety of the ligand binds to the primary combining site of VVLB4 in a similar way as observed for other Gal/GalNAc-specific plant lectins. The amino acid moiety of the Tn antigen is largely exposed to the solvent and makes few contacts with the protein. The structure of the complex provides a framework to understand the differences in the strength of VVLB4 binding to different sugars and emphasizes the role of a single protein residue, Tyr127, as a structural determinant of Tn-binding specificity.

About this Structure

Full crystallographic information is available from OCA.

Reference

The crystal structure of a plant lectin in complex with the Tn antigen., Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM, FEBS Lett. 2003 Feb 11;536(1-3):106-10. PMID:12586347 Page seeded by OCA on Sat May 3 02:04:10 2008

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