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| | <StructureSection load='5t5c' size='340' side='right'caption='[[5t5c]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='5t5c' size='340' side='right'caption='[[5t5c]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5t5c]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5T5C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5t5c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/DNA_launch_vector_pDE-GFP2 DNA launch vector pDE-GFP2] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T5C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.851Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t3v|5t3v]], [[5t40|5t40]], [[5t4i|5t4i]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EXOG, ENDOGL1, ENDOGL2, ENGL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5c OCA], [https://pdbe.org/5t5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t5c RCSB], [https://www.ebi.ac.uk/pdbsum/5t5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5c ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5t5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5c OCA], [http://pdbe.org/5t5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5c RCSB], [http://www.ebi.ac.uk/pdbsum/5t5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5c ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EXOG_HUMAN EXOG_HUMAN]] Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.<ref>PMID:18187503</ref> | + | [https://www.uniprot.org/uniprot/EXOG_HUMAN EXOG_HUMAN] Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.<ref>PMID:18187503</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: DNA launch vector pDE-GFP2]] |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Szymanski, M R]] | + | [[Category: Szymanski MR]] |
| - | [[Category: Yin, W Y]] | + | [[Category: Yin WY]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Dna-repair]]
| + | |
| - | [[Category: Exonuclease]]
| + | |
| - | [[Category: Hydrolase-dna complex]]
| + | |
| - | [[Category: Mitochondria]]
| + | |
| Structural highlights
Function
EXOG_HUMAN Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.[1]
Publication Abstract from PubMed
Human EXOG (hEXOG) is a 5'-exonuclease that is crucial for mitochondrial DNA repair; the enzyme belongs to a nonspecific nuclease family that includes the apoptotic endonuclease EndoG. Here we report biochemical and structural studies of hEXOG, including structures in its apo form and in a complex with DNA at 1.81 and 1.85 A resolution, respectively. A Wing domain, absent in other betabetaalpha-Me members, suppresses endonuclease activity, but confers on hEXOG a strong 5'-dsDNA exonuclease activity that precisely excises a dinucleotide using an intrinsic 'tape-measure'. The symmetrical apo hEXOG homodimer becomes asymmetrical upon binding to DNA, providing a structural basis for how substrate DNA bound to one active site allosterically regulates the activity of the other. These properties of hEXOG suggest a pathway for mitochondrial BER that provides an optimal substrate for subsequent gap-filling synthesis by DNA polymerase gamma.
A domain in human EXOG converts apoptotic endonuclease to DNA-repair exonuclease.,Szymanski MR, Yu W, Gmyrek AM, White MA, Molineux IJ, Lee JC, Yin YW Nat Commun. 2017 May 3;8:14959. doi: 10.1038/ncomms14959. PMID:28466855[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cymerman IA, Chung I, Beckmann BM, Bujnicki JM, Meiss G. EXOG, a novel paralog of Endonuclease G in higher eukaryotes. Nucleic Acids Res. 2008 Mar;36(4):1369-79. doi: 10.1093/nar/gkm1169. Epub 2008, Jan 10. PMID:18187503 doi:http://dx.doi.org/10.1093/nar/gkm1169
- ↑ Szymanski MR, Yu W, Gmyrek AM, White MA, Molineux IJ, Lee JC, Yin YW. A domain in human EXOG converts apoptotic endonuclease to DNA-repair exonuclease. Nat Commun. 2017 May 3;8:14959. doi: 10.1038/ncomms14959. PMID:28466855 doi:http://dx.doi.org/10.1038/ncomms14959
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