1n6q

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[[Image:1n6q.gif|left|200px]]
[[Image:1n6q.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1n6q |SIZE=350|CAPTION= <scene name='initialview01'>1n6q</scene>, resolution 3.&Aring;
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The line below this paragraph, containing "STRUCTURE_1n6q", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ATM:3&#39;-AZIDO-3&#39;-DEOXYTHYMIDINE-5&#39;-MONOPHOSPHATE'>ATM</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MRG:N2-(3-MERCAPTOPROPYL)-2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>MRG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
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-->
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|DOMAIN=
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{{STRUCTURE_1n6q| PDB=1n6q | SCENE= }}
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|RELATEDENTRY=[[1n5y|1N5Y]], [[1rtd|1RTD]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6q OCA], [http://www.ebi.ac.uk/pdbsum/1n6q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n6q RCSB]</span>
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}}
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'''HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)'''
'''HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)'''
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[[Category: Sayer, J M.]]
[[Category: Sayer, J M.]]
[[Category: Tuske, S.]]
[[Category: Tuske, S.]]
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[[Category: complex n]]
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[[Category: Complex n]]
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[[Category: drug resistance]]
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[[Category: Drug resistance]]
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[[Category: hiv]]
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[[Category: Hiv]]
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[[Category: nucleotide excision]]
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[[Category: Nucleotide excision]]
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[[Category: pre-translocation]]
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[[Category: Pre-translocation]]
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[[Category: reverse transcriptase]]
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[[Category: Reverse transcriptase]]
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[[Category: translocation]]
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[[Category: Translocation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:09:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:10 2008''
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Revision as of 23:09, 2 May 2008

Image:1n6q.gif

Template:STRUCTURE 1n6q

HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)


Overview

AZT (3'-azido-3'-deoxythymidine) resistance involves the enhanced excision of AZTMP from the end of the primer strand by HIV-1 reverse transcriptase. This reaction can occur when an AZTMP-terminated primer is bound at the nucleotide-binding site (pre-translocation complex N) but not at the 'priming' site (post-translocation complex P). We determined the crystal structures of N and P complexes at 3.0 and 3.1 A resolution. These structures provide insight into the structural basis of AZTMP excision and the mechanism of translocation. Docking of a dNTP in the P complex structure suggests steric crowding in forming a stable ternary complex that should increase the relative amount of the N complex, which is the substrate for excision. Structural differences between complexes N and P suggest that the conserved YMDD loop is involved in translocation, acting as a springboard that helps to propel the primer terminus from the N to the P site after dNMP incorporation.

About this Structure

1N6Q is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structures of HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA., Sarafianos SG, Clark AD Jr, Das K, Tuske S, Birktoft JJ, Ilankumaran P, Ramesha AR, Sayer JM, Jerina DM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2002 Dec 2;21(23):6614-24. PMID:12456667 Page seeded by OCA on Sat May 3 02:09:59 2008

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OCA

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