5w0v
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5w0v is ON HOLD until May 31 2019 Authors: An, S., Yoon, J., Song, J.-J., Cho, U.-S. Description: Crystal structure of full-length Kluyveromyces la...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34== | |
| + | <StructureSection load='5w0v' size='340' side='right'caption='[[5w0v]], [[Resolution|resolution]] 2.82Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W0V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.821Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [https://pdbe.org/5w0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [https://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6CMF0_KLULA Q6CMF0_KLULA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation. | ||
| - | + | Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123.,An S, Yoon J, Kim H, Song JJ, Cho US Elife. 2017 Oct 16;6. pii: e30244. doi: 10.7554/eLife.30244. PMID:29035199<ref>PMID:29035199</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: An | + | <div class="pdbe-citations 5w0v" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Yoon | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Kluyveromyces lactis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: An S]] | ||
| + | [[Category: Cho U-S]] | ||
| + | [[Category: Song J-J]] | ||
| + | [[Category: Yoon J]] | ||
Current revision
Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34
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