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| | <StructureSection load='5weg' size='340' side='right'caption='[[5weg]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='5weg' size='340' side='right'caption='[[5weg]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5weg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharum_hybrid_cultivar_sp-80-3280 Saccharum hybrid cultivar sp-80-3280]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5weg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharum_hybrid_cultivar_SP80-3280 Saccharum hybrid cultivar SP80-3280]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WEG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ugpase-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=193079 Saccharum hybrid cultivar SP-80-3280])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UTP--glucose-1-phosphate_uridylyltransferase UTP--glucose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.9 2.7.7.9] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5weg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weg OCA], [https://pdbe.org/5weg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5weg RCSB], [https://www.ebi.ac.uk/pdbsum/5weg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5weg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5weg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weg OCA], [http://pdbe.org/5weg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weg RCSB], [http://www.ebi.ac.uk/pdbsum/5weg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A075E2Q1_9POAL A0A075E2Q1_9POAL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Saccharum hybrid cultivar sp-80-3280]] | + | [[Category: Saccharum hybrid cultivar SP80-3280]] |
| - | [[Category: UTP--glucose-1-phosphate uridylyltransferase]]
| + | [[Category: Cotrim CA]] |
| - | [[Category: Cotrim, C A]] | + | [[Category: Kobe B]] |
| - | [[Category: Kobe, B]] | + | [[Category: Menossi M]] |
| - | [[Category: Menossi, M]] | + | [[Category: Soares JSM]] |
| - | [[Category: Soares, J S.M]] | + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
A0A075E2Q1_9POAL
Publication Abstract from PubMed
UDP-glucose pyrophosphorylase (UGPase) is found in all organisms and catalyses the formation of UDP-glucose. In sugarcane, UDP-glucose is a branch-point in the carbon channelling into other carbohydrates, such as sucrose and cellulose, which are the major factors for sugarcane productivity. In most plants, UGPase has been described to be enzymatically active in the monomeric form, while in human and yeast, homo-octamers represent the active form of the protein. Here, we present the crystal structure of UGPase from sugarcane (ScUGPase-1) at resolution of 2.0 A. The crystals of ScUGPase-1 reveal the presence of two molecules in the asymmetric unit and the multi-angle light scattering analysis shows that ScUGPase-1 forms a mixture of species ranging from monomers to larger oligomers in solution, suggesting similarities with the orthologs from yeast and human.
Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane.,Cotrim CA, Soares JSM, Kobe B, Menossi M PLoS One. 2018 Mar 1;13(3):e0193667. doi: 10.1371/journal.pone.0193667., eCollection 2018. PMID:29494650[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cotrim CA, Soares JSM, Kobe B, Menossi M. Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane. PLoS One. 2018 Mar 1;13(3):e0193667. doi: 10.1371/journal.pone.0193667., eCollection 2018. PMID:29494650 doi:http://dx.doi.org/10.1371/journal.pone.0193667
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