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6b9p

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'''Unreleased structure'''
 
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The entry 6b9p is ON HOLD until Paper Publication
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==Structure of GH 38 Jack Bean alpha-mannosidase in complex with a 36-valent iminosugar cluster inhibitor==
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<StructureSection load='6b9p' size='340' side='right'caption='[[6b9p]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6b9p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B9P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.996&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=D0J:(2R,3R,4R,5S)-2-(hydroxymethyl)-1-{9-[4-(methoxymethyl)-1H-1,2,3-triazol-1-yl]nonyl}piperidine-3,4,5-triol'>D0J</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b9p OCA], [https://pdbe.org/6b9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b9p RCSB], [https://www.ebi.ac.uk/pdbsum/6b9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b9p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MANA_CANEN MANA_CANEN] Liberates mannose from p-nitrophenyl-alpha-D-mannoside (PubMed:12325362, PubMed:4973951, PubMed:1156387). Liberates mannose from further alpha-D-mannosides including methyl-, benzyl-alpha-D-mannoside, 1-6-linked di-, tri- and tetrasaccharides of alpha-D-mannose and mannosyl-rhamnose (PubMed:12325362). Liberates mannose from various glycoproteins like ovalbumin and ovomucoid (PubMed:12325362, PubMed:5145). Does not hydrolyze beta-D-mannosides (PubMed:12325362). Has glycosyltransferase activity, forming disaccharides from mannose and lyxose but not from glucose, galactose, ribose, xylose or arabinose (PubMed:12325362).<ref>PMID:1156387</ref> <ref>PMID:12325362</ref> <ref>PMID:4973951</ref> <ref>PMID:5145</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Multivalent design of glycosidase inhibitors is a promising strategy for the treatment of diseases involving enzymatic hydrolysis of glycosidic bonds in carbohydrates. An essential prerequisite for successful applications is the atomic-level understanding of how outstanding binding enhancement occurs with multivalent inhibitors. Herein we report the first high-resolution crystal structures of the Jack bean alpha-mannosidase (JBalpha-man) in apo and inhibited states. The three-dimensional structure of JBalpha-man in complex with the multimeric cyclopeptoid-based inhibitor displaying the largest binding enhancements reported so far provides decisive insight into the molecular mechanisms underlying multivalent effects in glycosidase inhibition.
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Authors: Howard, E., Cousido-Siah, A., Lepage, M., Bodlenner, A., Mitschler, A., Meli, A., De Riccardis, F., Izzo, I., Podjarny, A., Compain, P.
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Structural Basis of Outstanding Multivalent Effects in Jack Bean alpha-Mannosidase Inhibition.,Howard E, Cousido-Siah A, Lepage ML, Schneider JP, Bodlenner A, Mitschler A, Meli A, Izzo I, Alvarez HA, Podjarny A, Compain P Angew Chem Int Ed Engl. 2018 Jul 2;57(27):8002-8006. doi: 10.1002/anie.201801202., Epub 2018 Jun 6. PMID:29722924<ref>PMID:29722924</ref>
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Description: Structure of GH 38 Jack Bean alpha-mannosidase in complex with a 36-valent iminosugar cluster inhibitor
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Izzo, I]]
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<div class="pdbe-citations 6b9p" style="background-color:#fffaf0;"></div>
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[[Category: Mitschler, A]]
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[[Category: Lepage, M]]
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==See Also==
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[[Category: De Riccardis, F]]
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
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[[Category: Podjarny, A]]
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== References ==
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[[Category: Bodlenner, A]]
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<references/>
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[[Category: Cousido-Siah, A]]
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__TOC__
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[[Category: Compain, P]]
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</StructureSection>
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[[Category: Howard, E]]
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[[Category: Canavalia ensiformis]]
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[[Category: Meli, A]]
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[[Category: Large Structures]]
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[[Category: Bodlenner A]]
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[[Category: Compain P]]
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[[Category: Cousido-Siah A]]
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[[Category: De Riccardis F]]
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[[Category: Howard E]]
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[[Category: Izzo I]]
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[[Category: Lepage M]]
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[[Category: Meli A]]
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[[Category: Mitschler A]]
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[[Category: Podjarny A]]

Current revision

Structure of GH 38 Jack Bean alpha-mannosidase in complex with a 36-valent iminosugar cluster inhibitor

PDB ID 6b9p

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