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1n96

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[[Image:1n96.gif|left|200px]]
[[Image:1n96.gif|left|200px]]
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{{Structure
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|PDB= 1n96 |SIZE=350|CAPTION= <scene name='initialview01'>1n96</scene>
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The line below this paragraph, containing "STRUCTURE_1n96", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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{{STRUCTURE_1n96| PDB=1n96 | SCENE= }}
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|RELATEDENTRY=[[1eu2|1EU2]], [[1eu6|1EU6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n96 OCA], [http://www.ebi.ac.uk/pdbsum/1n96 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n96 RCSB]</span>
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'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)'''
'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)'''
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==About this Structure==
==About this Structure==
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1N96 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA].
==Reference==
==Reference==
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12733903 12733903]
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12733903 12733903]
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[[Category: Protein complex]]
 
[[Category: Escaja, N.]]
[[Category: Escaja, N.]]
[[Category: Gelpi, J L.]]
[[Category: Gelpi, J L.]]
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[[Category: Pedroso, E.]]
[[Category: Pedroso, E.]]
[[Category: Rico, M.]]
[[Category: Rico, M.]]
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[[Category: bi-loop]]
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[[Category: Bi-loop]]
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[[Category: four-stranded dna]]
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[[Category: Four-stranded dna]]
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[[Category: quadruplex]]
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[[Category: Quadruplex]]
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[[Category: unusual dna]]
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[[Category: Unusual dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:15:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:27:09 2008''
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Revision as of 23:15, 2 May 2008

Template:STRUCTURE 1n96

DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)


Overview

The solution structure of a cyclic oligonucleotide d<pCGCTCATT> has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition.

About this Structure

Full crystallographic information is available from OCA.

Reference

Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:12733903 Page seeded by OCA on Sat May 3 02:15:06 2008

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