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6brm

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The crystal structure of isothiocyanate hydrolase from Delia radicum gut bacteria

Structural highlights

6brm is a 8 chain structure with sequence from Pectobacterium carotovorum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.55Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0N7FW12_PECCA

Publication Abstract from PubMed

Isothiocyanates (ITCs) are produced by cruciferous plants to protect them against herbivores and infection by microbes. These compounds are of particular interest due to their antimicrobial and anticarcinogenic properties. The breakdown of ITCs in nature is catalyzed by isothiocyanate hydrolase (ITCase), a novel family within the metallo-beta-lactamase-fold superfamily of proteins. saxA genes that code for ITCases are particularly widespread in insect- and plant-associated bacteria. Enzymatic characterization of seven phylogenetically related but distinct ITCases exhibited similar activities on six selected ITCs, suggesting that phylogenetic diversity does not determine the substrate specificity of ITCases. X-ray crystallography studies of two ITCases sharing 42% amino acid sequence identity revealed a highly conserved tertiary structure. Notable features of ITCase include a hydrophobic active site with two Zn(2+) ions coordinating water/hydroxide, and a flexible cap that is implicated in substrate recognition that covers the active site. This report reveals the function and structure of the previously uncharacterized family of isothiocyanate hydrolases within the otherwise relatively well-studied superfamily of metallo-beta-lactamases.Importance: This study explores a newly discovered protein in the beta-lactamase superfamily: SaxA, or isothiocyanate hydrolase. Isothiocyanates are defensive compounds found in many cabbage-related crop plants, and are currently being investigated for their antimicrobial and anticarcinogenic properties. We show that isothiocyanate hydrolases are responsible for the breakdown of several of these plant defensive chemicals in vitro and suggest their potential for mitigating the beneficial effects of isothiocyanates in crop protection and cancer prevention.

Functional profiling and crystal structures of isothiocyanate hydrolases found in gut-associated and plant pathogenic bacteria.,van den Bosch TJM, Tan K, Joachimiak A, Welte CU Appl Environ Microbiol. 2018 May 11. pii: AEM.00478-18. doi:, 10.1128/AEM.00478-18. PMID:29752272^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ van den Bosch TJM, Tan K, Joachimiak A, Welte CU. Functional profiling and crystal structures of isothiocyanate hydrolases found in gut-associated and plant pathogenic bacteria. Appl Environ Microbiol. 2018 May 11. pii: AEM.00478-18. doi:, 10.1128/AEM.00478-18. PMID:29752272 doi:http://dx.doi.org/10.1128/AEM.00478-18

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6brm"

@@ Line 3: / Line 3: @@
 <StructureSection load='6brm' size='340' side='right'caption='[[6brm]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6brm]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BRM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6brm]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BRM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6brm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6brm OCA], [http://pdbe.org/6brm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6brm RCSB], [http://www.ebi.ac.uk/pdbsum/6brm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6brm ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6brm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6brm OCA], [https://pdbe.org/6brm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6brm RCSB], [https://www.ebi.ac.uk/pdbsum/6brm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6brm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0N7FW12_PECCA A0A0N7FW12_PECCA]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus carotovorus jones 1901]]
 [[Category: Large Structures]]
-[[Category: Bosch, T van den]]
+[[Category: Pectobacterium carotovorum]]
-[[Category: Joachimiak, A]]
+[[Category: Joachimiak A]]
-[[Category: Tan, K]]
+[[Category: Tan K]]
-[[Category: Welte, C]]
+[[Category: Welte C]]
-[[Category: Drgb3_saxa]]
+[[Category: Van den Bosch T]]
-[[Category: Hydrolase]]
-[[Category: Isothiocyanate hydrolase]]
-[[Category: Itc]]
-[[Category: Saxa]]

6brm

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Current revision

The crystal structure of isothiocyanate hydrolase from Delia radicum gut bacteria

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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