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6c8s

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Loganic acid methyltransferase with SAH

Structural highlights

6c8s is a 2 chain structure with sequence from Catharanthus roseus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LAMT_CATRO Component of the seco-iridoid and derivatives monoterpenoid indole alkaloids (MIAs, e.g. vinblastine and ajmalicine) biosynthesis pathway. Catalyzes the methylation of loganic acid (6S,7R) to produce loganin (PubMed:29399933, PubMed:18326827, PubMed:24104568). Weak activity with secologanic acid as substrate. Inactive on deoxyloganic, dehydrologanic, epiloganic and loganetic acid (PubMed:18326827).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Loganin is an iridoid glycoside of interest as both an intermediate in the biosynthesis of indole alkaloids in plants and as a bioactive compound itself. The loganic acid methyltransferase catalyzes the methylation of a monoterpenoid glycoside precursor to produce loganin, and demonstrates stereospecificity for the (6S,7R) substrate. Here, we biochemically characterize this biocatalyst and elucidate the basis for its strict substrate specificity. These studies may help facilitate the design of new classes of monoterpenoid indole alkaloids of pharmaceutical interest.

Loganic Acid Methyltransferase: Insights into the specificity of methylation on an iridoid glycoside.,Petronikolou N, Hollatz A, Schuler MA, Nair SK Chembiochem. 2018 Feb 5. doi: 10.1002/cbic.201700679. PMID:29399933^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murata J, Roepke J, Gordon H, De Luca V. The leaf epidermome of Catharanthus roseus reveals its biochemical specialization. Plant Cell. 2008 Mar;20(3):524-42. PMID:18326827 doi:10.1105/tpc.107.056630
↑ Asada K, Salim V, Masada-Atsumi S, Edmunds E, Nagatoshi M, Terasaka K, Mizukami H, De Luca V. A 7-deoxyloganetic acid glucosyltransferase contributes a key step in secologanin biosynthesis in Madagascar periwinkle. Plant Cell. 2013 Oct;25(10):4123-34. PMID:24104568 doi:10.1105/tpc.113.115154
↑ Petronikolou N, Hollatz A, Schuler MA, Nair SK. Loganic Acid Methyltransferase: Insights into the specificity of methylation on an iridoid glycoside. Chembiochem. 2018 Feb 5. doi: 10.1002/cbic.201700679. PMID:29399933 doi:http://dx.doi.org/10.1002/cbic.201700679
↑ Petronikolou N, Hollatz A, Schuler MA, Nair SK. Loganic Acid Methyltransferase: Insights into the specificity of methylation on an iridoid glycoside. Chembiochem. 2018 Feb 5. doi: 10.1002/cbic.201700679. PMID:29399933 doi:http://dx.doi.org/10.1002/cbic.201700679

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6c8s"

Categories: Catharanthus roseus | Large Structures | Nair SK | Petronikolou N

@@ Line 3: / Line 3: @@
 <StructureSection load='6c8s' size='340' side='right'caption='[[6c8s]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6c8s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Catro Catro]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6C8S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6c8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catharanthus_roseus Catharanthus roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C8S FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6c8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c8s OCA], [http://pdbe.org/6c8s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c8s RCSB], [http://www.ebi.ac.uk/pdbsum/6c8s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c8s ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c8s OCA], [https://pdbe.org/6c8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c8s RCSB], [https://www.ebi.ac.uk/pdbsum/6c8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c8s ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LAMT_CATRO LAMT_CATRO] Component of the seco-iridoid and derivatives monoterpenoid indole alkaloids (MIAs, e.g. vinblastine and ajmalicine) biosynthesis pathway. Catalyzes the methylation of loganic acid (6S,7R) to produce loganin (PubMed:29399933, PubMed:18326827, PubMed:24104568). Weak activity with secologanic acid as substrate. Inactive on deoxyloganic, dehydrologanic, epiloganic and loganetic acid (PubMed:18326827).<ref>PMID:18326827</ref> <ref>PMID:24104568</ref> <ref>PMID:29399933</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Catro]]
+[[Category: Catharanthus roseus]]
 [[Category: Large Structures]]
-[[Category: Nair, S K]]
+[[Category: Nair SK]]
-[[Category: Petronikolou, N]]
+[[Category: Petronikolou N]]
-[[Category: Loganic acid]]
-[[Category: Methyltransferase]]
-[[Category: Plant protein]]
-[[Category: Transferase]]

6c8s

From Proteopedia

Current revision

Loganic acid methyltransferase with SAH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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