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| | <StructureSection load='6dbg' size='340' side='right'caption='[[6dbg]], [[Resolution|resolution]] 1.51Å' scene=''> | | <StructureSection load='6dbg' size='340' side='right'caption='[[6dbg]], [[Resolution|resolution]] 1.51Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dbg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932] and [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dbg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DBG FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6dba|6dba]], [[6dbd|6dbd]], [[6dbf|6dbf]], [[6dbe|6dbe]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">inlB, lmo0434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dbg OCA], [https://pdbe.org/6dbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dbg RCSB], [https://www.ebi.ac.uk/pdbsum/6dbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dbg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dbg OCA], [http://pdbe.org/6dbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dbg RCSB], [http://www.ebi.ac.uk/pdbsum/6dbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dbg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/INLB_LISMO INLB_LISMO] Mediates the entry of Listeria monocytogenes into cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium monocytogenes hominis nyfeldt 1932]] | + | [[Category: Camelus dromedarius]] |
| - | [[Category: Arabian camel]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Brooks, C L]] | + | [[Category: Listeria monocytogenes]] |
| - | [[Category: Huh, I]] | + | [[Category: Brooks CL]] |
| - | [[Category: King, M Toride]] | + | [[Category: Huh I]] |
| - | [[Category: Immune system]] | + | [[Category: Toride King M]] |
| - | [[Category: Nanobody vhh listeria internalin b]]
| + | |
| Structural highlights
Function
INLB_LISMO Mediates the entry of Listeria monocytogenes into cells.
Publication Abstract from PubMed
Listeria monocytogenes causes Listeriosis, a potentially fatal food borne disease. The condition is especially harmful to pregnant women. Listeria outbreaks can originate from diverse foods, highlighting the need for novel strategies to improve food safety. The first step in Listeria invasion is internalization of the bacteria, which is mediated by the interaction of the internalin family of virulence factors with host cell receptors. A crucial interaction for Listeria invasion of the placenta, and thus is a target for therapeutic intervention, is between Internalin B (InlB) and the receptor c-Met.. Single-domain antibodies (VHH, also called nanobodies, or sdAbs) from camel heavy-chain antibodies are a novel solution for preventing Listeria infections. The VHH R303, R330 and R326 all bind InlB with high affinity, however the molecular mechanism behind their mode of action was unknown. We demonstrate that despite a high degree of sequence and structural diversity, the VHH bind a single epitope on InlB. A combination of gentamicin protection assays and florescent microscopy establish that InlB specific VHH inhibit Listeria invasion of HeLa cells. A high resolution X-ray structure of VHH R303 in complex with InlB showed that the VHH binds at the c-Met interaction site on InlB, thereby acting as a competitive inhibitor preventing bacterial invasion. These results point to the potential of VHH as a novel class of therapeutics for the prevention of Listeriosis.
Structural Basis of VHH mediated neutralization of the foodborne pathogen Listeria monocytogenes.,Toride King M, Huh I, Shenai A, Brooks TM, Brooks CL J Biol Chem. 2018 Jul 5. pii: RA118.003888. doi: 10.1074/jbc.RA118.003888. PMID:29976754[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Toride King M, Huh I, Shenai A, Brooks TM, Brooks CL. Structural Basis of VHH mediated neutralization of the foodborne pathogen Listeria monocytogenes. J Biol Chem. 2018 Jul 5. pii: RA118.003888. doi: 10.1074/jbc.RA118.003888. PMID:29976754 doi:http://dx.doi.org/10.1074/jbc.RA118.003888
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