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3awk

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[[Image:3awk.jpg|left|200px]]
 
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==Crystal structure of the polyketide synthase 1 from huperzia serrata==
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The line below this paragraph, containing "STRUCTURE_3awk", creates the "Structure Box" on the page.
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<StructureSection load='3awk' size='340' side='right'caption='[[3awk]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3awk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Huperzia_serrata Huperzia serrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AWK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3awk| PDB=3awk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awk OCA], [https://pdbe.org/3awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3awk RCSB], [https://www.ebi.ac.uk/pdbsum/3awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3awk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A3E7Z7_HUPSR A3E7Z7_HUPSR]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HsPKS1 from Huperzia serrata is a type III polyketide synthase (PKS) with remarkable substrate tolerance and catalytic potential. Here we present the synthesis of unnatural unique polyketide-alkaloid hybrid molecules by exploiting the enzyme reaction using precursor-directed and structure-based approaches. HsPKS1 produced novel pyridoisoindole (or benzopyridoisoindole) with the 6.5.6-fused (or 6.6.5.6-fused) ring system by the condensation of 2-carbamoylbenzoyl-CoA (or 3-carbamoyl-2-naphthoyl-CoA), a synthetic nitrogen-containing nonphysiological starter substrate, with two molecules of malonyl-CoA. The structure-based S348G mutant not only extended the product chain length but also altered the cyclization mechanism to produce a biologically active, ring-expanded 6.7.6-fused dibenzoazepine, by the condensation of 2-carbamoylbenzoyl-CoA with three malonyl-CoAs. Thus, the basic nitrogen atom and the structure-based mutagenesis enabled additional C horizontal line C and C horizontal line N bond formation to generate the novel polyketide-alkaloid scaffold.
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===Crystal structure of the polyketide synthase 1 from huperzia serrata===
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Synthesis of unnatural alkaloid scaffolds by exploiting plant polyketide synthase.,Morita H, Yamashita M, Shi SP, Wakimoto T, Kondo S, Kato R, Sugio S, Kohno T, Abe I Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13504-9. Epub 2011 Aug 8. PMID:21825160<ref>PMID:21825160</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3awk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21825160 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21825160}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3awk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Huperzia_serrata Huperzia serrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWK OCA].
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==Reference==
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<ref group="xtra">PMID:021825160</ref><references group="xtra"/>
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[[Category: Huperzia serrata]]
[[Category: Huperzia serrata]]
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[[Category: Abe, I.]]
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[[Category: Large Structures]]
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[[Category: Kato, R.]]
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[[Category: Abe I]]
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[[Category: Kohno, T.]]
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[[Category: Kato R]]
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[[Category: Kondo, S.]]
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[[Category: Kohno T]]
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[[Category: Morita, H.]]
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[[Category: Kondo S]]
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[[Category: Sugio, S.]]
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[[Category: Morita H]]
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[[Category: Chalcone synthase]]
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[[Category: Sugio S]]
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[[Category: Transferase]]
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[[Category: Type iii polyketide synthase]]
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Current revision

Crystal structure of the polyketide synthase 1 from huperzia serrata

PDB ID 3awk

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