This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1nb4
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1nb4.jpg|left|200px]] | [[Image:1nb4.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1nb4", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1nb4| PDB=1nb4 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''HC-J4 RNA polymerase apo-form''' | '''HC-J4 RNA polymerase apo-form''' | ||
| Line 30: | Line 27: | ||
[[Category: Rowlands, D J.]] | [[Category: Rowlands, D J.]] | ||
[[Category: Trowbridge, R.]] | [[Category: Trowbridge, R.]] | ||
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: De-novo priming]] |
| - | [[Category: | + | [[Category: Function analysis]] |
| - | [[Category: | + | [[Category: Hcv]] |
| - | [[Category: | + | [[Category: Hepatitis c virus]] |
| - | [[Category: | + | [[Category: Ns5b]] |
| - | [[Category: | + | [[Category: Rdrp]] |
| - | [[Category: | + | [[Category: Replication]] |
| - | [[Category: | + | [[Category: Rna polymerase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:19:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:19, 2 May 2008
HC-J4 RNA polymerase apo-form
Overview
Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases.
About this Structure
1NB4 is a Single protein structure of sequence from Hepatitis c virus. Full crystallographic information is available from OCA.
Reference
Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:12589751 Page seeded by OCA on Sat May 3 02:19:11 2008
