6dgd
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6dgd is ON HOLD Authors: Satyshur, K.A., Windgassen, T.A., Keck, J.L. Description: PriA helicase bound to dsDNA of a DNA replication fork [[Categor...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==PriA helicase bound to dsDNA of a DNA replication fork== | |
| + | <StructureSection load='6dgd' size='340' side='right'caption='[[6dgd]], [[Resolution|resolution]] 2.82Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6dgd]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DGD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.823Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgd OCA], [https://pdbe.org/6dgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dgd RCSB], [https://www.ebi.ac.uk/pdbsum/6dgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1W2ITH4_KLEPN A0A1W2ITH4_KLEPN] Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.[HAMAP-Rule:MF_00983] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA replication restart, the essential process that reinitiates prematurely terminated genome replication reactions, relies on exquisitely specific recognition of abandoned DNA replication-fork structures. The PriA DNA helicase mediates this process in bacteria through mechanisms that remain poorly defined. We report the crystal structure of a PriA/replication-fork complex, which resolves leading-strand duplex DNA bound to the protein. Interaction with PriA unpairs one end of the DNA and sequesters the 3'-most nucleotide from the nascent leading strand into a conserved protein pocket. Cross-linking studies reveal a surface on the winged-helix domain of PriA that binds to parental duplex DNA. Deleting the winged-helix domain alters PriA's structure-specific DNA unwinding properties and impairs its activity in vivo. Our observations lead to a model in which coordinated parental-, leading-, and lagging-strand DNA binding provide PriA with the structural specificity needed to act on abandoned DNA replication forks. | ||
| - | + | Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase.,Windgassen TA, Leroux M, Satyshur KA, Sandler SJ, Keck JL Proc Natl Acad Sci U S A. 2018 Sep 10. pii: 1809842115. doi:, 10.1073/pnas.1809842115. PMID:30201718<ref>PMID:30201718</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6dgd" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Satyshur | + | ==See Also== |
| + | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Klebsiella pneumoniae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Keck JL]] | ||
| + | [[Category: Satyshur KA]] | ||
| + | [[Category: Windgassen TA]] | ||
Current revision
PriA helicase bound to dsDNA of a DNA replication fork
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