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6ow4

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Structure of the NADH-bound form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32

Structural highlights

6ow4 is a 8 chain structure with sequence from Bifidobacterium adolescentis L2-32. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.99Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A7A7R9_BIFAD

Publication Abstract from PubMed

Anaerobic bacteria inhabiting the human gastrointestinal tract have evolved various enzymes that modify host-derived steroids. The bacterial steroid-17,20-desmolase pathway cleaves the cortisol side-chain, forming pro-androgens predicted to impact host physiology. Bacterial 20beta-hydroxysteroid dehydrogenase (20beta-HSDH) regulates cortisol side-chain cleavage by reducing the C-20 carboxyl group on cortisol, yielding 20beta-dihydrocortisol. Recently, the gene encoding 20beta-HSDH in Butyricicoccus desmolans ATCC 43058 was reported, and a non-redundant protein search yielded a candidate 20beta-HSDH gene in Bifidobacterium adolescentis strain L2-32. B. adolescentis 20beta-HSDH could regulate cortisol side-chain cleavage by limiting pro-androgen formation in bacteria such as Clostridium scindens and 21-dehydroxylation by Eggerthella lenta Here, the putative B. adolescentis 20beta-HSDH was cloned, overexpressed and purified. 20beta-HSDH activity was confirmed through whole cell and pure enzymatic assays and it is specific for cortisol. Next, we solved the structures of recombinant 20beta-HSDH in both the apo and holo forms at 2.0-2.2A resolutions, revealing close overlap except for rearrangements near the active site. Interestingly, the structures contain a large, flexible N-terminal region which was investigated by gel filtration chromatography and CD spectroscopy. This extended N-terminus is important for protein stability because deletions of varying lengths caused structural changes and reduced enzymatic activity. A nonconserved extended N-terminus was also observed in several short-chain dehydro-genase/reductase family members. B. adolescentis strains capable of 20beta-HSDH activity could alter glucocorticoid metabolism in the gut and thereby serve as potential probiotics for the management of androgen-dependent diseases.

Structural and biochemical characterization of 20beta-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.,Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM J Biol Chem. 2019 Jun 17. pii: RA119.009390. doi: 10.1074/jbc.RA119.009390. PMID:31209107^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM. Structural and biochemical characterization of 20beta-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32. J Biol Chem. 2019 Jun 17. pii: RA119.009390. doi: 10.1074/jbc.RA119.009390. PMID:31209107 doi:http://dx.doi.org/10.1074/jbc.RA119.009390

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ow4"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ow4' size='340' side='right'caption='[[6ow4]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ow4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_adolescentis_l2-32 Bifidobacterium adolescentis l2-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OW4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ow4]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_adolescentis_L2-32 Bifidobacterium adolescentis L2-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OW4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BIFADO_01909 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=411481 Bifidobacterium adolescentis L2-32])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ow4 OCA], [http://pdbe.org/6ow4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ow4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ow4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ow4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ow4 OCA], [https://pdbe.org/6ow4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ow4 RCSB], [https://www.ebi.ac.uk/pdbsum/6ow4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ow4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A7A7R9_BIFAD A7A7R9_BIFAD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 6ow4" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bifidobacterium adolescentis l2-32]]
+[[Category: Bifidobacterium adolescentis L2-32]]
 [[Category: Large Structures]]
-[[Category: Koropatkin, N M]]
+[[Category: Koropatkin NM]]
-[[Category: Mythen, S M]]
+[[Category: Mythen SM]]
-[[Category: Pollet, R M]]
+[[Category: Pollet RM]]
-[[Category: Ridlon, J M]]
+[[Category: Ridlon JM]]
-[[Category: Nadh dependent]]
-[[Category: Oxidoreductase]]
-[[Category: Pyridine nucleotide-dependent enzyme]]
-[[Category: Short-chain dehydrogenase/reductase]]

6ow4

From Proteopedia

Current revision

Structure of the NADH-bound form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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