6phb

From Proteopedia

(Difference between revisions)

Current revision

Pfs25 in complex with the human transmission blocking antibody 2530

Structural highlights

6phb is a 6 chain structure with sequence from Homo sapiens and Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OS25_PLAFO

Publication Abstract from PubMed

Transmission-blocking vaccines have the potential to be key contributors to malaria elimination. Such vaccines elicit antibodies that inhibit parasites during their development in Anopheles mosquitoes, thus breaking the cycle of transmission. To date, characterization of humoral responses to Plasmodium falciparum transmission-blocking vaccine candidate Pfs25 has largely been conducted in pre-clinical models. Here, we present molecular analyses of human antibody responses generated in a clinical trial evaluating Pfs25 vaccination. From a collection of monoclonal antibodies with transmission-blocking activity, we identify the most potent transmission-blocking antibody yet described against Pfs25; 2544. The interactions of 2544 and three other antibodies with Pfs25 are analyzed by crystallography to understand structural requirements for elicitation of human transmission-blocking responses. Our analyses provide insights into Pfs25 immunogenicity and epitope potency, and detail an affinity maturation pathway for a potent transmission-blocking antibody in humans. Our findings can be employed to guide the design of improved malaria transmission-blocking vaccines.

Potent antibody lineage against malaria transmission elicited by human vaccination with Pfs25.,McLeod B, Miura K, Scally SW, Bosch A, Nguyen N, Shin H, Kim D, Volkmuth W, Ramisch S, Chichester JA, Streatfield S, Woods C, Schief WR, Emerling D, King CR, Julien JP Nat Commun. 2019 Sep 24;10(1):4328. doi: 10.1038/s41467-019-11980-6. PMID:31551421^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McLeod B, Miura K, Scally SW, Bosch A, Nguyen N, Shin H, Kim D, Volkmuth W, Ramisch S, Chichester JA, Streatfield S, Woods C, Schief WR, Emerling D, King CR, Julien JP. Potent antibody lineage against malaria transmission elicited by human vaccination with Pfs25. Nat Commun. 2019 Sep 24;10(1):4328. doi: 10.1038/s41467-019-11980-6. PMID:31551421 doi:http://dx.doi.org/10.1038/s41467-019-11980-6

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6phb"

Categories: Homo sapiens | Large Structures | Plasmodium falciparum | Julien JP | McLeod BR

@@ Line 3: / Line 3: @@
 <StructureSection load='6phb' size='340' side='right'caption='[[6phb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6phb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PHB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6phb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PHB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6phb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6phb OCA], [http://pdbe.org/6phb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6phb RCSB], [http://www.ebi.ac.uk/pdbsum/6phb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6phb ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6phb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6phb OCA], [https://pdbe.org/6phb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6phb RCSB], [https://www.ebi.ac.uk/pdbsum/6phb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6phb ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/OS25_PLAFO OS25_PLAFO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 19: @@
 </div>
 <div class="pdbe-citations 6phb" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[3D structures of human antibody|3D structures of human antibody]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Plafa]]
+[[Category: Plasmodium falciparum]]
-[[Category: Julien, J P]]
+[[Category: Julien JP]]
-[[Category: McLeod, B R]]
+[[Category: McLeod BR]]
-[[Category: Antigen]]
-[[Category: Complex]]
-[[Category: Fab]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin]]

6phb

From Proteopedia

Current revision

Pfs25 in complex with the human transmission blocking antibody 2530

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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