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6u2m
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635== | |
| + | <StructureSection load='6u2m' size='340' side='right'caption='[[6u2m]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6u2m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U2M FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PUJ:(1E,3S)-1-{10-[2-carboxy-5-({2-[2-(hexyloxy)ethoxy]ethyl}carbamoyl)phenyl]-7-(3-fluoroazetidin-1-yl)-5,5-dimethyldibenz+o[b,e]silin-3(5H)-ylidene}-3-fluoroazetidin-1-ium'>PUJ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u2m OCA], [https://pdbe.org/6u2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u2m RCSB], [https://www.ebi.ac.uk/pdbsum/6u2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u2m ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Functional imaging using fluorescent indicators has revolutionized biology, but additional sensor scaffolds are needed to access properties such as bright, far-red emission. Here, we introduce a new platform for 'chemigenetic' fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. We solve a crystal structure of HaloTag bound to a rhodamine dye ligand to guide engineering efforts to modulate the dye environment. We show that fusion of HaloTag with protein sensor domains that undergo conformational changes near the bound dye results in large and rapid changes in fluorescence output. This generalizable approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in cultured neurons. | ||
| - | + | The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.,Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER Nat Chem Biol. 2021 Apr 1. pii: 10.1038/s41589-021-00775-w. doi:, 10.1038/s41589-021-00775-w. PMID:33795886<ref>PMID:33795886</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Deo | + | <div class="pdbe-citations 6u2m" style="background-color:#fffaf0;"></div> |
| - | [[Category: Schreiter | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rhodococcus sp]] | ||
| + | [[Category: Deo C]] | ||
| + | [[Category: Schreiter ER]] | ||
Current revision
Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635
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