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5ler
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the bacterial sex F pilus (13.2 Angstrom rise)== | |
| + | <SX load='5ler' size='340' side='right' viewer='molstar' caption='[[5ler]], [[Resolution|resolution]] 5.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ler]] is a 75 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LER FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6V6:[(2~{S})-3-[[(2~{R})-2,3-BIS(OXIDANYL)PROPOXY]-OXIDANYL-PHOSPHORYL]OXY-2-HEXADEC-9-ENOYLOXY-PROPYL]+HEXADECANOATE'>6V6</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ler FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ler OCA], [https://pdbe.org/5ler PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ler RCSB], [https://www.ebi.ac.uk/pdbsum/5ler PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ler ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PIL1_ECOLI PIL1_ECOLI] Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (PubMed:1464628, PubMed:6090426). Functions as a receptor for CdiA-CT from E.cloacae and E.coli, although it is not clear if this is physiologically relevant (PubMed:24889811).<ref>PMID:1464628</ref> <ref>PMID:24889811</ref> <ref>PMID:6090426</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 A resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection. | ||
| - | + | Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.,Costa TR, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G Cell. 2016 Sep 8;166(6):1436-1444.e10. doi: 10.1016/j.cell.2016.08.025. PMID:27610568<ref>PMID:27610568</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5ler" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Costa TRD]] | ||
| + | [[Category: Egelman EH]] | ||
| + | [[Category: Ilangovan I]] | ||
| + | [[Category: Redzej A]] | ||
| + | [[Category: Santini JM]] | ||
| + | [[Category: Smith TK]] | ||
| + | [[Category: Ukleja M]] | ||
| + | [[Category: Waksman G]] | ||
Current revision
Structure of the bacterial sex F pilus (13.2 Angstrom rise)
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Categories: Escherichia coli | Large Structures | Costa TRD | Egelman EH | Ilangovan I | Redzej A | Santini JM | Smith TK | Ukleja M | Waksman G
