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7jri
From Proteopedia
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==High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle, 33 ms structure== | ==High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle, 33 ms structure== | ||
| - | <StructureSection load='7jri' size='340' side='right'caption='[[7jri]]' scene=''> | + | <StructureSection load='7jri' size='340' side='right'caption='[[7jri]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JRI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7jri]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stigmatella_aurantiaca Stigmatella aurantiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JRI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jri OCA], [https://pdbe.org/7jri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jri RCSB], [https://www.ebi.ac.uk/pdbsum/7jri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jri ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3Q8:3-[2-[[5-[[(3E,4S)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1H-pyrrol-2-yl]methyl]-5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-4-methyl-1H-pyrrol-3-yl]propanoic+acid'>3Q8</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jri OCA], [https://pdbe.org/7jri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jri RCSB], [https://www.ebi.ac.uk/pdbsum/7jri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jri ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q09E27_STIAD Q09E27_STIAD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting Pr state and the light-activated Pfr state. Light signals are transduced as structural change through the entire protein to modulate its activity. It is unknown how the Pr-to-Pfr interconversion occurs, as the structure of intermediates remains notoriously elusive. Here, we present short-lived crystal structures of the photosensory core modules of the bacteriophytochrome from myxobacterium Stigmatella aurantiaca captured by an X-ray free electron laser 5 ns and 33 ms after light illumination of the Pr state. We observe large structural displacements of the covalently bound bilin chromophore, which trigger a bifurcated signaling pathway that extends through the entire protein. The snapshots show with atomic precision how the signal progresses from the chromophore, explaining how plants, bacteria, and fungi sense red light. | ||
| + | |||
| + | High-resolution crystal structures of transient intermediates in the phytochrome photocycle.,Carrillo M, Pandey S, Sanchez J, Noda M, Poudyal I, Aldama L, Malla TN, Claesson E, Wahlgren WY, Feliz D, Srajer V, Maj M, Castillon L, Iwata S, Nango E, Tanaka R, Tanaka T, Fangjia L, Tono K, Owada S, Westenhoff S, Stojkovic EA, Schmidt M Structure. 2021 Mar 20. pii: S0969-2126(21)00077-0. doi:, 10.1016/j.str.2021.03.004. PMID:33756101<ref>PMID:33756101</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7jri" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Stigmatella aurantiaca]] | ||
[[Category: Schmidt M]] | [[Category: Schmidt M]] | ||
[[Category: Stojkovic E]] | [[Category: Stojkovic E]] | ||
Current revision
High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle, 33 ms structure
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