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| | <StructureSection load='7rdb' size='340' side='right'caption='[[7rdb]], [[Resolution|resolution]] 2.52Å' scene=''> | | <StructureSection load='7rdb' size='340' side='right'caption='[[7rdb]], [[Resolution|resolution]] 2.52Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7rdb]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RDB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7rdb]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RDB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7rd5|7rd5]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rdb OCA], [https://pdbe.org/7rdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rdb RCSB], [https://www.ebi.ac.uk/pdbsum/7rdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rdb ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rdb OCA], [https://pdbe.org/7rdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rdb RCSB], [https://www.ebi.ac.uk/pdbsum/7rdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rdb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TSN15_HUMAN TSN15_HUMAN]] Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862, PubMed:30463011). Promotes ADAM10-mediated cleavage of CDH2 (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).[UniProtKB:F7BWT7]<ref>PMID:26686862</ref> <ref>PMID:30463011</ref>
| + | [https://www.uniprot.org/uniprot/TSN15_HUMAN TSN15_HUMAN] Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862, PubMed:30463011). Promotes ADAM10-mediated cleavage of CDH2 (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).[UniProtKB:F7BWT7]<ref>PMID:26686862</ref> <ref>PMID:30463011</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blacklow, S C]] | + | [[Category: Blacklow SC]] |
| - | [[Category: Durr, K L]] | + | [[Category: Durr KL]] |
| - | [[Category: Gabriel, K H]] | + | [[Category: Gabriel KH]] |
| - | [[Category: Lipper, C H]] | + | [[Category: Lipper CH]] |
| - | [[Category: Seegar, T C.M]] | + | [[Category: Seegar TCM]] |
| - | [[Category: Tomlinson, M G]] | + | [[Category: Tomlinson MG]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Protein trafficking]]
| + | |
| - | [[Category: Tetraspanin]]
| + | |
| Structural highlights
Function
TSN15_HUMAN Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862, PubMed:30463011). Promotes ADAM10-mediated cleavage of CDH2 (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).[UniProtKB:F7BWT7][1] [2]
Publication Abstract from PubMed
Tetraspanins are four-pass transmembrane proteins that function by regulating trafficking of partner proteins and organizing signaling complexes in the membrane. Tspan15, one of a six-member TspanC8 subfamily, forms a complex that regulates the trafficking, maturation, and substrate selectivity of the transmembrane protease ADAM10, an essential enzyme in mammalian physiology that cleaves a wide variety of membrane-anchored substrates, including Notch receptors, amyloid precursor protein, cadherins, and growth factors. We present here crystal structures of the Tspan15 large extracellular loop (LEL) required for functional association with ADAM10 both in isolation and in complex with the Fab fragment of an anti-Tspan15 antibody. Comparison of the Tspan15 LEL with other tetraspanin LEL structures shows that a core helical framework buttresses a variable region that structurally diverges among LELs. Using co-immunoprecipitation and a cellular N-cadherin cleavage assay, we identify a site on Tspan15 required for both ADAM10 binding and promoting substrate cleavage.
Crystal structure of the Tspan15 LEL domain reveals a conserved ADAM10 binding site.,Lipper CH, Gabriel KH, Seegar TCM, Durr KL, Tomlinson MG, Blacklow SC Structure. 2021 Nov 2. pii: S0969-2126(21)00375-0. doi:, 10.1016/j.str.2021.10.007. PMID:34739841[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jouannet S, Saint-Pol J, Fernandez L, Nguyen V, Charrin S, Boucheix C, Brou C, Milhiet PE, Rubinstein E. TspanC8 tetraspanins differentially regulate the cleavage of ADAM10 substrates, Notch activation and ADAM10 membrane compartmentalization. Cell Mol Life Sci. 2016 May;73(9):1895-915. doi: 10.1007/s00018-015-2111-z. Epub , 2015 Dec 19. PMID:26686862 doi:http://dx.doi.org/10.1007/s00018-015-2111-z
- ↑ Shah J, Rouaud F, Guerrera D, Vasileva E, Popov LM, Kelley WL, Rubinstein E, Carette JE, Amieva MR, Citi S. A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote alpha-Toxin Cytotoxicity. Cell Rep. 2018 Nov 20;25(8):2132-2147.e7. doi: 10.1016/j.celrep.2018.10.088. PMID:30463011 doi:http://dx.doi.org/10.1016/j.celrep.2018.10.088
- ↑ Lipper CH, Gabriel KH, Seegar TCM, Durr KL, Tomlinson MG, Blacklow SC. Crystal structure of the Tspan15 LEL domain reveals a conserved ADAM10 binding site. Structure. 2021 Nov 2. pii: S0969-2126(21)00375-0. doi:, 10.1016/j.str.2021.10.007. PMID:34739841 doi:http://dx.doi.org/10.1016/j.str.2021.10.007
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