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2x31

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Current revision (18:15, 18 October 2023) (edit) (undo)
 
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[[Image:2x31.png|left|200px]]
 
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==Modelling of the complex between subunits BchI and BchD of magnesium chelatase based on single-particle cryo-EM reconstruction at 7.5 ang==
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The line below this paragraph, containing "STRUCTURE_2x31", creates the "Structure Box" on the page.
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<SX load='2x31' size='340' side='right' viewer='molstar' caption='[[2x31]], [[Resolution|resolution]] 7.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x31]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X31 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x31 OCA], [https://pdbe.org/2x31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x31 RCSB], [https://www.ebi.ac.uk/pdbsum/2x31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x31 ProSAT]</span></td></tr>
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{{STRUCTURE_2x31| PDB=2x31 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BCHD_RHOCB BCHD_RHOCB] Involved in bacteriochlorophyll biosynthesis; introduces a magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mg-chelatase catalyzes the first committed step of the chlorophyll biosynthetic pathway, the ATP-dependent insertion of Mg(2+) into protoporphyrin IX (PPIX). Here we report the reconstruction using single-particle cryo-electron microscopy of the complex between subunits BchD and BchI of Rhodobacter capsulatus Mg-chelatase in the presence of ADP, the nonhydrolyzable ATP analog AMPPNP, and ATP at 7.5 A, 14 A, and 13 A resolution, respectively. We show that the two AAA+ modules of the subunits form a unique complex of 3 dimers related by a three-fold axis. The reconstructions demonstrate substantial differences between the conformations of the complex in the presence of ATP and ADP, and suggest that the C-terminal integrin-I domains of the BchD subunits play a central role in transmitting conformational changes of BchI to BchD. Based on these data a model for the function of magnesium chelatase is proposed.
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===Modelling of the complex between subunits BchI and BchD of magnesium chelatase based on single-particle cryo-EM reconstruction at 7.5 ang===
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ATP-induced conformational dynamics in the AAA+ motor unit of magnesium chelatase.,Lundqvist J, Elmlund H, Wulff RP, Berglund L, Elmlund D, Emanuelsson C, Hebert H, Willows RD, Hansson M, Lindahl M, Al-Karadaghi S Structure. 2010 Mar 10;18(3):354-65. PMID:20223218<ref>PMID:20223218</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20223218}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x31" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20223218 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20223218}}
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Large Structures]]
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[[2x31]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X31 OCA].
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==Reference==
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<ref group="xtra">PMID:020223218</ref><references group="xtra"/>
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[[Category: Magnesium chelatase]]
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[[Category: Rhodobacter capsulatus]]
[[Category: Rhodobacter capsulatus]]
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[[Category: Al-Karadaghi, S.]]
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[[Category: Al-Karadaghi S]]
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[[Category: Berglund, L.]]
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[[Category: Berglund L]]
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[[Category: Elmlund, D.]]
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[[Category: Elmlund D]]
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[[Category: Elmlund, H.]]
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[[Category: Elmlund H]]
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[[Category: Emanuelsson, C.]]
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[[Category: Emanuelsson C]]
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[[Category: Hansson, M.]]
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[[Category: Hansson M]]
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[[Category: Hebert, H.]]
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[[Category: Hebert H]]
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[[Category: Lindahl, M.]]
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[[Category: Lindahl M]]
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[[Category: Lunqvist, J.]]
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[[Category: Lunqvist J]]
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[[Category: Willows, R D.]]
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[[Category: Peterson Wulff R]]
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[[Category: Wulff, R Peterson.]]
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[[Category: Willows RD]]
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[[Category: Bacteriochlorophyll biosynthesis]]
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[[Category: Ligase]]
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[[Category: Photosynthesis]]
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Current revision

Modelling of the complex between subunits BchI and BchD of magnesium chelatase based on single-particle cryo-EM reconstruction at 7.5 ang

2x31, resolution 7.50Å

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