1nns
From Proteopedia
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'''L-asparaginase of E. coli in C2 space group and 1.95 A resolution''' | '''L-asparaginase of E. coli in C2 space group and 1.95 A resolution''' | ||
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[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
[[Category: Sanches, M.]] | [[Category: Sanches, M.]] | ||
| - | [[Category: | + | [[Category: Amidrohydrolase]] |
| - | [[Category: | + | [[Category: Crystallographic comparison]] |
| - | [[Category: | + | [[Category: L-asparaginase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:45:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:45, 2 May 2008
L-asparaginase of E. coli in C2 space group and 1.95 A resolution
Overview
The functional L-asparaginase from Escherichia coli is a homotetramer with a molecular weight of about 142 kDa. The X-ray structure of the enzyme, crystallized in a new form (space group C2) and refined to 1.95 A resolution, is compared with that of the previously determined crystal form (space group P2(1)). The asymmetric unit of the new crystal form contains an L-asparaginase dimer instead of the tetramer found in the previous crystal form. It is found that crystal contacts practically do not affect the conformation of the protein. It is shown that subunit C of the tetrameric form is in a conformation which is systematically different from that of all other subunits in both crystal forms. Major conformational differences are confined to the lid loop (residues 14-27). In addition, the stability of this globular protein is analyzed in terms of the interactions between hydrophobic parts of the subunits.
About this Structure
1NNS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups., Sanches M, Barbosa JA, de Oliveira RT, Abrahao Neto J, Polikarpov I, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):416-22. Epub 2003, Feb 21. PMID:12595697 Page seeded by OCA on Sat May 3 02:45:29 2008
