1v45

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(New page: 200px<br /> <applet load="1v45" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v45, resolution 2.86&Aring;" /> '''Crystal Structure o...)
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[[Image:1v45.gif|left|200px]]<br />
 
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<applet load="1v45" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1v45, resolution 2.86&Aring;" />
 
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'''Crystal Structure of human PNP complexed with 3-deoxyguanosine'''<br />
 
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==Overview==
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==Crystal Structure of human PNP complexed with 3-deoxyguanosine==
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Purine nucleoside phosphorylase (PNP) is a key enzyme in the, purine-salvage pathway, which allows cells to utilize preformed bases and, nucleosides in order to synthesize nucleotides. PNP is specific for purine, nucleosides in the beta-configuration and exhibits a strong preference for, purines containing a 6-keto group and ribosyl-containing nucleosides, relative to the corresponding analogues. PNP was crystallized in complex, with ligands and data collection was performed using synchrotron, radiation. This work reports the structure of human PNP in complex with, guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution), and 8-azaguanine (at 2.85 A resolution). These structures were compared, with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved, previously.
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<StructureSection load='1v45' size='340' side='right'caption='[[1v45]], [[Resolution|resolution]] 2.86&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1v45]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V45 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DG:9-(3-DEOXY-BETA-D-RIBOFURANOSYL)GUANINE'>3DG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v45 OCA], [https://pdbe.org/1v45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v45 RCSB], [https://www.ebi.ac.uk/pdbsum/1v45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v45 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/PNPH_HUMAN PNPH_HUMAN] Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNPD) [MIM:[https://omim.org/entry/613179 613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.<ref>PMID:3029074</ref> <ref>PMID:1384322</ref> <ref>PMID:8931706</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/PNPH_HUMAN PNPH_HUMAN] The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.<ref>PMID:2104852</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/1v45_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v45 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.
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==Disease==
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Structure of human PNP complexed with ligands.,Canduri F, Silva RG, dos Santos DM, Palma MS, Basso LA, Santos DS, de Azevedo WF Jr Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):856-62. Epub 2005, Jun 24. PMID:15983407<ref>PMID:15983407</ref>
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Known diseases associated with this structure: Neutral lipid storage disease with myopathy OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=609059 609059]], Nucleoside phosphorylase deficiency, immunodeficiency due to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=164050 164050]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1V45 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and 3DG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V45 OCA].
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</div>
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<div class="pdbe-citations 1v45" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure of human PNP complexed with ligands., Canduri F, Silva RG, dos Santos DM, Palma MS, Basso LA, Santos DS, de Azevedo WF Jr, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):856-62. Epub 2005, Jun 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15983407 15983407]
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*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Purine-nucleoside phosphorylase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Basso LA]]
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[[Category: Basso, L.A.]]
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[[Category: Canduri F]]
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[[Category: Canduri, F.]]
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[[Category: De Azevedo Jr WF]]
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[[Category: Jr., W.F.De.Azevedo.]]
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[[Category: Dos Santos DM]]
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[[Category: Mendes, M.A.]]
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[[Category: Mendes MA]]
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[[Category: Palma, M.S.]]
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[[Category: Palma MS]]
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[[Category: Santos, D.M.Dos.]]
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[[Category: Santos DS]]
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[[Category: Santos, D.S.]]
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[[Category: Silva RG]]
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[[Category: Silva, R.G.]]
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[[Category: 3DG]]
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[[Category: SO4]]
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[[Category: crystallography]]
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[[Category: drug design]]
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[[Category: purine nucleoside phosphorylase]]
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[[Category: synchrotron]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:41:19 2007''
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Current revision

Crystal Structure of human PNP complexed with 3-deoxyguanosine

PDB ID 1v45

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