1vfv

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(New page: 200px<br /><applet load="1vfv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vfv, resolution 1.85&Aring;" /> '''Crystal Structure of...)
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[[Image:1vfv.gif|left|200px]]<br /><applet load="1vfv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vfv, resolution 1.85&Aring;" />
 
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'''Crystal Structure of the Kif1A Motor Domain Complexed With Mg-AMPPNP'''<br />
 
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==Overview==
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==Crystal Structure of the Kif1A Motor Domain Complexed With Mg-AMPPNP==
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The motor protein kinesin moves along microtubules, driven by adenosine, triphosphate (ATP) hydrolysis. However, it remains unclear how kinesin, converts the chemical energy into mechanical movement. We report crystal, structures of monomeric kinesin KIF1A with three transition-state analogs:, adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes). These structures, together with, known structures of the ADP-bound state and the, adenylyl-(beta,gamma-methylene) diphosphate (AMP-PCP)-bound state, show, that kinesin uses two microtubule-binding loops in an alternating manner, to change its interaction with microtubules during the ATP hydrolysis, cycle; loop L11 is extended in the AMP-PNP structure, whereas loop L12 is, extended in the ADP structure. ADP-vanadate displays an intermediate, structure in which a conformational change in two switch regions causes, both loops to be raised from the microtubule, thus actively detaching, kinesin.
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<StructureSection load='1vfv' size='340' side='right'caption='[[1vfv]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vfv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VFV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vfv OCA], [https://pdbe.org/1vfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vfv RCSB], [https://www.ebi.ac.uk/pdbsum/1vfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vfv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KIF1A_MOUSE KIF1A_MOUSE] Motor for anterograde axonal transport of synaptic vesicle precursors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/1vfv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vfv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The motor protein kinesin moves along microtubules, driven by adenosine triphosphate (ATP) hydrolysis. However, it remains unclear how kinesin converts the chemical energy into mechanical movement. We report crystal structures of monomeric kinesin KIF1A with three transition-state analogs: adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes). These structures, together with known structures of the ADP-bound state and the adenylyl-(beta,gamma-methylene) diphosphate (AMP-PCP)-bound state, show that kinesin uses two microtubule-binding loops in an alternating manner to change its interaction with microtubules during the ATP hydrolysis cycle; loop L11 is extended in the AMP-PNP structure, whereas loop L12 is extended in the ADP structure. ADP-vanadate displays an intermediate structure in which a conformational change in two switch regions causes both loops to be raised from the microtubule, thus actively detaching kinesin.
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==About this Structure==
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KIF1A alternately uses two loops to bind microtubules.,Nitta R, Kikkawa M, Okada Y, Hirokawa N Science. 2004 Jul 30;305(5684):678-83. PMID:15286375<ref>PMID:15286375</ref>
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1VFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VFV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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KIF1A alternately uses two loops to bind microtubules., Nitta R, Kikkawa M, Okada Y, Hirokawa N, Science. 2004 Jul 30;305(5684):678-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15286375 15286375]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1vfv" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Hirokawa, N.]]
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[[Category: Kikkawa, M.]]
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[[Category: Nitta, R.]]
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[[Category: Okada, Y.]]
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[[Category: ANP]]
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[[Category: MG]]
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[[Category: kinesin]]
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[[Category: microtubule]]
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[[Category: motor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:48:34 2007''
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==See Also==
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Hirokawa N]]
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[[Category: Kikkawa M]]
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[[Category: Nitta R]]
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[[Category: Okada Y]]

Current revision

Crystal Structure of the Kif1A Motor Domain Complexed With Mg-AMPPNP

PDB ID 1vfv

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