2a3c

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{{Seed}}
 
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[[Image:2a3c.png|left|200px]]
 
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==Crystal structure of Aspergillus fumigatus chitinase B1 in complex with pentoxifylline==
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The line below this paragraph, containing "STRUCTURE_2a3c", creates the "Structure Box" on the page.
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<StructureSection load='2a3c' size='340' side='right'caption='[[2a3c]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2a3c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3C FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNX:3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>PNX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2a3c| PDB=2a3c | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3c OCA], [https://pdbe.org/2a3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3c RCSB], [https://www.ebi.ac.uk/pdbsum/2a3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHIB1_ASPFM CHIB1_ASPFM] Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Family 18 chitinases play key roles in a range of pathogenic organisms and are overexpressed in the asthmatic lung. By screening a library of marketed drug molecules, we have identified methylxanthine derivatives as possible inhibitor leads. These derivatives, theophylline, caffeine, and pentoxifylline, are used therapeutically as antiinflammatory agents, with pleiotropic mechanisms of action. Here it is shown that they are also competitive inhibitors against a fungal family 18 chitinase, with pentoxifylline being the most potent (K(i) of 37 microM). Crystallographic analysis of chitinase-inhibitor complexes revealed specific interactions with the active site, mimicking the reaction intermediate analog, allosamidin. Mutagenesis identified the key active site residues, conserved in mammalian chitinases, which contribute to inhibitor affinity. Enzyme assays also revealed that these methylxanthines are active against human chitinases.
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===Crystal structure of Aspergillus fumigatus chitinase B1 in complex with pentoxifylline===
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Methylxanthine drugs are chitinase inhibitors: investigation of inhibition and binding modes.,Rao FV, Andersen OA, Vora KA, Demartino JA, van Aalten DM Chem Biol. 2005 Sep;12(9):973-80. PMID:16183021<ref>PMID:16183021</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2a3c" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16183021}}, adds the Publication Abstract to the page
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16183021 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16183021}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2A3C is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3C OCA].
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==Reference==
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<ref group="xtra">PMID:16183021</ref><references group="xtra"/>
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[[Category: Aspergillus fumigatus]]
[[Category: Aspergillus fumigatus]]
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[[Category: Chitinase]]
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[[Category: Large Structures]]
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[[Category: Aalten, D M.F van.]]
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[[Category: Andersen OA]]
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[[Category: Andersen, O A.]]
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[[Category: DeMartino JA]]
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[[Category: DeMartino, J A.]]
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[[Category: Rao FV]]
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[[Category: Rao, F V.]]
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[[Category: Vora KA]]
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[[Category: Vora, K A.]]
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[[Category: Van Aalten DMF]]
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[[Category: Chitinase-pentoxifylline complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:19:01 2009''
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Current revision

Crystal structure of Aspergillus fumigatus chitinase B1 in complex with pentoxifylline

PDB ID 2a3c

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