2cw0

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[[Image:2cw0.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution==
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|PDB= 2cw0 |SIZE=350|CAPTION= <scene name='initialview01'>2cw0</scene>, resolution 3.3&Aring;
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<StructureSection load='2cw0' size='340' side='right'caption='[[2cw0]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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<table><tr><td colspan='2'>[[2cw0]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus], [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CW0 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cw0 OCA], [https://pdbe.org/2cw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2cw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cw0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cw0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
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'''Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution'''
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Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation.,Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E Cell. 2005 Aug 26;122(4):541-52. PMID:16122422<ref>PMID:16122422</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cw0" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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==About this Structure==
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== References ==
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2CW0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW0 OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation., Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E, Cell. 2005 Aug 26;122(4):541-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16122422 16122422]
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[[Category: Large Structures]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Protein complex]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Arnold, E.]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Birktoft, J J.]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Borukhov, S.]]
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[[Category: Arnold E]]
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[[Category: Dharia, C.]]
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[[Category: Birktoft JJ]]
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[[Category: Ebright, R H.]]
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[[Category: Borukhov S]]
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[[Category: Hudson, B.]]
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[[Category: Clark Jr AD]]
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[[Category: Ismail, S.]]
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[[Category: Dharia C]]
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[[Category: Jr., A D.Clark.]]
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[[Category: Ebright RH]]
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[[Category: Laptenko, O.]]
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[[Category: Hudson B]]
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[[Category: Lee, J.]]
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[[Category: Ismail S]]
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[[Category: Leroy, O.]]
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[[Category: Laptenko O]]
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[[Category: Mukhopadhyay, J.]]
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[[Category: Lee J]]
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[[Category: Napoli, A.]]
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[[Category: Leroy O]]
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[[Category: Sarafianos, S G.]]
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[[Category: Mukhopadhyay J]]
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[[Category: Sineva, E.]]
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[[Category: Napoli A]]
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[[Category: Tuske, S.]]
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[[Category: Sarafianos SG]]
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[[Category: Wang, X.]]
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[[Category: Sineva E]]
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[[Category: MG]]
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[[Category: Tuske S]]
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[[Category: ZN]]
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[[Category: Wang X]]
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[[Category: bent-bridge helix]]
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[[Category: rna polymerase holoenzyme]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:20:25 2008''
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Current revision

Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution

PDB ID 2cw0

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