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2d22

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==Crystal structure of covalent glycosyl-enzyme intermediate of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86==
==Crystal structure of covalent glycosyl-enzyme intermediate of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86==
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<StructureSection load='2d22' size='340' side='right' caption='[[2d22]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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<StructureSection load='2d22' size='340' side='right'caption='[[2d22]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2d22]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_olivaceoviridis"_preobrazhenskaya_and_ryabova_in_gauze_et_al._1957 "actinomyces olivaceoviridis" preobrazhenskaya and ryabova in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D22 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2d22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_olivaceoviridis Streptomyces olivaceoviridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D22 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d1z|2d1z]], [[1xyf|1xyf]], [[1isw|1isw]], [[1isx|1isx]], [[2d20|2d20]], [[2d23|2d23]], [[2d24|2d24]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d22 OCA], [https://pdbe.org/2d22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d22 RCSB], [https://www.ebi.ac.uk/pdbsum/2d22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d22 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d22 OCA], [http://pdbe.org/2d22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d22 RCSB], [http://www.ebi.ac.uk/pdbsum/2d22 PDBsum]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7SI98_STROI Q7SI98_STROI]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/2d22_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/2d22_consurf.spt"</scriptWhenChecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d22 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Actinomyces olivaceoviridis preobrazhenskaya and ryabova in gauze et al. 1957]]
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[[Category: Large Structures]]
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Streptomyces olivaceoviridis]]
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[[Category: Fujimoto, Z]]
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[[Category: Fujimoto Z]]
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[[Category: Hasegawa, T]]
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[[Category: Hasegawa T]]
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[[Category: Ito, S]]
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[[Category: Ito S]]
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[[Category: Kaneko, S]]
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[[Category: Kaneko S]]
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[[Category: Kawahara, S I]]
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[[Category: Kawahara SI]]
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[[Category: Kuno, A]]
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[[Category: Kuno A]]
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[[Category: Suzuki, R]]
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[[Category: Suzuki R]]
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[[Category: Taira, K]]
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[[Category: Taira K]]
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[[Category: Catalytic-site mutant]]
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[[Category: Chemical rescue]]
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[[Category: Covalent glycosyl-enzyme intermediate]]
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[[Category: Hydrolase]]
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[[Category: Retaining enzyme]]
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[[Category: Tim-barrel]]
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Current revision

Crystal structure of covalent glycosyl-enzyme intermediate of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86

PDB ID 2d22

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