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2dep
From Proteopedia
(Difference between revisions)
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<StructureSection load='2dep' size='340' side='right'caption='[[2dep]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='2dep' size='340' side='right'caption='[[2dep]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2dep]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2dep]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoclostridium_stercorarium Thermoclostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DEP FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dep OCA], [https://pdbe.org/2dep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dep RCSB], [https://www.ebi.ac.uk/pdbsum/2dep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dep ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dep OCA], [https://pdbe.org/2dep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dep RCSB], [https://www.ebi.ac.uk/pdbsum/2dep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dep ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/CEXY_THEST CEXY_THEST] Active toward xylan, carboxymethylcellulose, P-nitrophenyl-beta-D-xylopyranoside and P-nitrophenyl-beta-D-cellobioside. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dep ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dep ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution. | ||
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| - | Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium.,Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719<ref>PMID:14747719</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2dep" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Atcc 35414]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fushinobu | + | [[Category: Thermoclostridium stercorarium]] |
| - | [[Category: Hayashi | + | [[Category: Fushinobu S]] |
| - | [[Category: Kitaoka | + | [[Category: Hayashi K]] |
| - | [[Category: Miyanaga | + | [[Category: Kitaoka M]] |
| - | [[Category: Nishimoto | + | [[Category: Miyanaga A]] |
| - | + | [[Category: Nishimoto M]] | |
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Current revision
Crystal Structure of xylanase B from Clostridium stercorarium F9
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