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2g9z

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{{Seed}}
 
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[[Image:2g9z.png|left|200px]]
 
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<!--
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==Thiamin pyrophosphokinase from Candida albicans==
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The line below this paragraph, containing "STRUCTURE_2g9z", creates the "Structure Box" on the page.
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<StructureSection load='2g9z' size='340' side='right'caption='[[2g9z]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2g9z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G9Z FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=VNP:3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-5-(2-{[HYDROXY(PHOSPHONOAMINO)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM'>VNP</scene></td></tr>
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{{STRUCTURE_2g9z| PDB=2g9z | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g9z OCA], [https://pdbe.org/2g9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g9z RCSB], [https://www.ebi.ac.uk/pdbsum/2g9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g9z ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/2g9z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g9z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: In search of new antifungal targets of potential interest for pharmaceutical companies, we initiated a comparative genomics study to identify the most promising protein-coding genes in fungal genomes. One criterion was the protein sequence conservation between reference pathogenic genomes. A second criterion was that the corresponding gene in Saccharomyces cerevisiae should be essential. Since thiamine pyrophosphate is an essential product involved in a variety of metabolic pathways, proteins responsible for its production satisfied these two criteria. RESULTS: We report the enzymatic characterization and the crystallographic structure of the Candida albicans Thiamine pyrophosphokinase. The protein was co-crystallized with thiamine or thiamine-PNP. CONCLUSION: The presence of an inorganic phosphate in the crystallographic structure opposite the known AMP binding site relative to the thiamine moiety suggests that a second AMP molecule could be accommodated in the C. albicans structure. Together with the crystallographic structures of the enzyme/substrate complexes this suggests the existence of a secondary, less specific, nucleotide binding site in the Candida albicans thiamine pyrophosphokinase which could transiently serve during the release or the binding of ATP. The structures also highlight a conserved Glutamine residue (Q138) which could interact with the ATP alpha-phosphate and act as gatekeeper. Finally, the TPK/Thiamine-PNP complex is consistent with a one step mechanism of pyrophosphorylation.
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===Thiamin pyrophosphokinase from Candida albicans===
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Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinase.,Santini S, Monchois V, Mouz N, Sigoillot C, Rousselle T, Claverie JM, Abergel C BMC Struct Biol. 2008 Jul 24;8:33. PMID:18652651<ref>PMID:18652651</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18652651}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2g9z" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18652651 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18652651}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2G9Z is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G9Z OCA].
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==Reference==
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<ref group="xtra">PMID:18652651</ref><references group="xtra"/>
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[[Category: Candida albicans]]
[[Category: Candida albicans]]
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[[Category: Thiamine diphosphokinase]]
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[[Category: Large Structures]]
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[[Category: Abergel, C.]]
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[[Category: Abergel C]]
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[[Category: Claverie, J M.]]
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[[Category: Claverie JM]]
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[[Category: IGS-CNRS, France BIGSBacterial targets at.]]
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[[Category: Monchois V]]
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[[Category: Monchois, V.]]
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[[Category: Rousselle T]]
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[[Category: Rousselle, T.]]
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[[Category: Santini S]]
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[[Category: Santini, S.]]
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[[Category: Bacterial targets at igs-cnr]]
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[[Category: Big]]
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[[Category: France]]
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[[Category: Profun]]
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[[Category: Structural genomic]]
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[[Category: Thiamin pyrophosphokinase]]
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[[Category: Thiamin-pnp]]
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[[Category: Tpk]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:54:32 2009''
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Current revision

Thiamin pyrophosphokinase from Candida albicans

PDB ID 2g9z

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