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2yrw
From Proteopedia
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| - | [[Image:2yrw.jpg|left|200px]]<br /><applet load="2yrw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2yrw, resolution 2.20Å" /> | ||
| - | '''Crystal structure of GAR synthetase from Geobacillus kaustophilus'''<br /> | ||
| - | == | + | ==Crystal structure of GAR synthetase from Geobacillus kaustophilus== |
| - | + | <StructureSection load='2yrw' size='340' side='right'caption='[[2yrw]], [[Resolution|resolution]] 2.20Å' scene=''> | |
| - | [ | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2yrw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YRW FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |
| - | [ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yrw OCA], [https://pdbe.org/2yrw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yrw RCSB], [https://www.ebi.ac.uk/pdbsum/2yrw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yrw ProSAT], [https://www.topsan.org/Proteins/RSGI/2yrw TOPSAN]</span></td></tr> | |
| - | [ | + | </table> |
| - | [[ | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/Q5L3C7_GEOKA Q5L3C7_GEOKA] | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | + | Check<jmol> | |
| - | [ | + | <jmolCheckbox> |
| - | [[ | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yr/2yrw_consurf.spt"</scriptWhenChecked> |
| - | [ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | + | <text>to colour the structure by Evolutionary Conservation</text> | |
| - | + | </jmolCheckbox> | |
| - | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2yrw ConSurf]. | |
| - | + | <div style="clear:both"></div> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
| - | + | Glycinamide ribonucleotide synthetase (GAR-syn, PurD) catalyses the second reaction of the purine biosynthetic pathway; the conversion of phosphoribosylamine, glycine and ATP to glycinamide ribonucleotide (GAR), ADP and Pi. In the present study, crystal structures of GAR-syn's from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus were determined in apo forms. Crystal structures in ligand-bound forms were also determined for G. kaustophilus and A. aeolicus proteins. In general, overall structures of GAR-syn's are similar to each other. However, the orientations of the B domains are varied among GAR-syn's and the MD simulation suggested the mobility of the B domain. Furthermore, it was demonstrated that the B loop in the B domain fixes the position of the beta- and gamma- phosphate groups of the bound ATP. The structures of GAR-syn's and the bound ligands were compared with each other in detail, and structures of GAR-syn's with full ligands, as well as the possible reaction mechanism, were proposed. | |
| - | + | Crystal structures of glycinamide ribonucleotide synthetase, PurD, from thermophilic eubacteria.,Sampei G, Baba S, Kanagawa M, Yanai H, Ishii T, Kawai H, Fukai Y, Ebihara A, Nakagawa N, Kawai G J Biochem. 2010 Oct;148(4):429-38. Epub 2010 Aug 16. PMID:20716513<ref>PMID:20716513</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2yrw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus kaustophilus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Baba S]] | ||
| + | [[Category: Kanagawa M]] | ||
| + | [[Category: Kawai G]] | ||
| + | [[Category: Kuramitsu S]] | ||
| + | [[Category: Sampei G]] | ||
| + | [[Category: Yokoyama S]] | ||
Current revision
Crystal structure of GAR synthetase from Geobacillus kaustophilus
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