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2ywf
From Proteopedia
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| - | [[Image:2ywf.png|left|200px]] | ||
| - | + | ==Crystal structure of GMPPNP-bound LepA from Aquifex aeolicus== | |
| - | + | <StructureSection load='2ywf' size='340' side='right'caption='[[2ywf]], [[Resolution|resolution]] 2.24Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2ywf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YWF FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24Å</td></tr> | |
| - | == | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | [[2ywf]] is a 1 chain structure with sequence from [ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ywf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ywf OCA], [https://pdbe.org/2ywf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ywf RCSB], [https://www.ebi.ac.uk/pdbsum/2ywf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ywf ProSAT], [https://www.topsan.org/Proteins/RSGI/2ywf TOPSAN]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LEPA_AQUAE LEPA_AQUAE] Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yw/2ywf_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ywf ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[GTP-binding protein|GTP-binding protein]] | + | *[[Elongation factor 3D structures|Elongation factor 3D structures]] |
| - | [[Category: Aquifex aeolicus | + | *[[Escherichia coli LepA%2C the ribosomal back translocase|Escherichia coli LepA%2C the ribosomal back translocase]] |
| - | [[Category: Hanawa-Suetsugu | + | *[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] |
| - | [[Category: Kaminishi | + | __TOC__ |
| - | [[Category: Kawazoe | + | </StructureSection> |
| - | [[Category: Nakayama-Ushikoshi | + | [[Category: Aquifex aeolicus VF5]] |
| - | [[Category: Nishino | + | [[Category: Large Structures]] |
| - | + | [[Category: Hanawa-Suetsugu K]] | |
| - | [[Category: Shirouzu | + | [[Category: Kaminishi T]] |
| - | [[Category: Takemoto | + | [[Category: Kawazoe M]] |
| - | [[Category: Terada | + | [[Category: Nakayama-Ushikoshi R]] |
| - | [[Category: Yokoyama | + | [[Category: Nishino A]] |
| - | + | [[Category: Shirouzu M]] | |
| - | + | [[Category: Takemoto C]] | |
| - | + | [[Category: Terada T]] | |
| - | + | [[Category: Yokoyama S]] | |
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Current revision
Crystal structure of GMPPNP-bound LepA from Aquifex aeolicus
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