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7lvu
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==Structure of RSV F-directed VHH Cl184== | ==Structure of RSV F-directed VHH Cl184== | ||
| - | <StructureSection load='7lvu' size='340' side='right'caption='[[7lvu]]' scene=''> | + | <StructureSection load='7lvu' size='340' side='right'caption='[[7lvu]], [[Resolution|resolution]] 1.94Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LVU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7lvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LVU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lvu OCA], [https://pdbe.org/7lvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lvu RCSB], [https://www.ebi.ac.uk/pdbsum/7lvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lvu ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lvu OCA], [https://pdbe.org/7lvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lvu RCSB], [https://www.ebi.ac.uk/pdbsum/7lvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lvu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human respiratory syncytial virus (RSV) is a major cause of lower respiratory tract disease, especially in young children and the elderly. The fusion protein (F) exists in a pre- and postfusion conformation and is the main target of RSV-neutralizing antibodies. Highly potent RSV-neutralizing antibodies typically bind sites that are unique to the prefusion conformation of F. In this study we screened a single-domain antibody (VHH) library derived from a llama immunized with prefusion-stabilized F and identified a prefusion F-specific VHH that can neutralize RSV A at subnanomolar concentrations. Structural analysis revealed that this VHH primarily binds to antigenic site I while also making contacts with residues in antigenic site III and IV. This new VHH reveals a previously underappreciated membrane-proximal region sensitive for neutralization.ImportanceRSV is an important respiratory pathogen. This study describes a prefusion F-specific VHH that primarily binds to antigenic site I of RSV F. This is the first time that a prefusion F-specific antibody that binds this site is reported. In general, antibodies that bind to site I are poorly neutralizing, whereas the VHH described here neutralizes RSV A at subnanomolar concentrations. Our findings contribute to insights into the RSV F antigenic map. | ||
| + | |||
| + | A vulnerable, membrane-proximal site in human respiratory syncytial virus F revealed by a prefusion-specific single-domain antibody.,Rossey I, Hsieh CL, Sedeyn K, Ballegeer M, Schepens B, Mclellan JS, Saelens X J Virol. 2021 Mar 10. pii: JVI.02279-20. doi: 10.1128/JVI.02279-20. PMID:33692208<ref>PMID:33692208</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lvu" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lama glama]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hsieh C-L]] | [[Category: Hsieh C-L]] | ||
[[Category: McLellan JS]] | [[Category: McLellan JS]] | ||
Current revision
Structure of RSV F-directed VHH Cl184
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