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8bt4
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8bt4 is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Ribonucleotide Reductase class Ie R2 from Mesoplasma florum, radical-lost ground state== | |
| + | <StructureSection load='8bt4' size='340' side='right'caption='[[8bt4]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8bt4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesoplasma_florum_L1 Mesoplasma florum L1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BT4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DAH:3,4-DIHYDROXYPHENYLALANINE'>DAH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bt4 OCA], [https://pdbe.org/8bt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bt4 RCSB], [https://www.ebi.ac.uk/pdbsum/8bt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bt4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6F0T5_MESFL Q6F0T5_MESFL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aerobic ribonucleotide reductases (RNRs) initiate synthesis of DNA building blocks by generating a free radical within the R2 subunit; the radical is subsequently shuttled to the catalytic R1 subunit through proton-coupled electron transfer (PCET). We present a high-resolution room temperature structure of the class Ie R2 protein radical captured by x-ray free electron laser serial femtosecond crystallography. The structure reveals conformational reorganization to shield the radical and connect it to the translocation path, with structural changes propagating to the surface where the protein interacts with the catalytic R1 subunit. Restructuring of the hydrogen bond network, including a notably short O-O interaction of 2.41 angstroms, likely tunes and gates the radical during PCET. These structural results help explain radical handling and mobilization in RNR and have general implications for radical transfer in proteins. | ||
| - | + | Structure of a ribonucleotide reductase R2 protein radical.,Lebrette H, Srinivas V, John J, Aurelius O, Kumar R, Lundin D, Brewster AS, Bhowmick A, Sirohiwal A, Kim IS, Gul S, Pham C, Sutherlin KD, Simon P, Butryn A, Aller P, Orville AM, Fuller FD, Alonso-Mori R, Batyuk A, Sauter NK, Yachandra VK, Yano J, Kaila VRI, Sjoberg BM, Kern J, Roos K, Hogbom M Science. 2023 Oct 6;382(6666):109-113. doi: 10.1126/science.adh8160. Epub 2023 , Oct 5. PMID:37797025<ref>PMID:37797025</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8bt4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mesoplasma florum L1]] | ||
| + | [[Category: Hogbom M]] | ||
| + | [[Category: Lebrette H]] | ||
| + | [[Category: Srinivas V]] | ||
Current revision
Ribonucleotide Reductase class Ie R2 from Mesoplasma florum, radical-lost ground state
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