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2zcg

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[[Image:2zcg.jpg|left|200px]]
 
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==Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum==
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The line below this paragraph, containing "STRUCTURE_2zcg", creates the "Structure Box" on the page.
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<StructureSection load='2zcg' size='340' side='right'caption='[[2zcg]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zcg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.223&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcg OCA], [https://pdbe.org/2zcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zcg RCSB], [https://www.ebi.ac.uk/pdbsum/2zcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zcg ProSAT]</span></td></tr>
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{{STRUCTURE_2zcg| PDB=2zcg | SCENE= }}
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</table>
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== Function ==
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'''Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum'''
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[https://www.uniprot.org/uniprot/Q8T6J6_PLAFA Q8T6J6_PLAFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zcg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zcg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum ( PfODCase, EC 4.1.1.23) has been overexpressed, purified, subjected to kinetic and biochemical analysis, and crystallized. The native enzyme is a homodimer with a subunit molecular mass of 38 kDa. The saturation curve for OMP as a substrate conformed to Michaelis-Menten kinetics with K m = 350 +/- 60 nM and V max = 2.70 +/- 0.10 micromol/min/mg protein. Inhibition patterns for nucleoside 5'-monophosphate analogues were linear competitive with respect to OMP with a decreasing potency of inhibition of PfODCase in the order: pyrazofurin 5'-monophosphate ( K i = 3.6 +/- 0.7 nM) &gt; xanthosine 5'-monophosphate (XMP, K i = 4.4 +/- 0.7 nM) &gt; 6-azauridine 5'-monophosphate (AzaUMP, K i = 12 +/- 3 nM) &gt; allopurinol-3-riboside 5'-monophosphate ( K i = 240 +/- 20 nM). XMP is an approximately 150-fold more potent inhibitor of PfODCase compared with the human enzyme. The structure of PfODCase was solved in the absence of ligand and displays a classic TIM-barrel fold characteristic of the enzyme. Both the phosphate-binding loop and the betaalpha5-loop have conformational flexibility, which may be associated with substrate capture and product release along the reaction pathway.
Orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum ( PfODCase, EC 4.1.1.23) has been overexpressed, purified, subjected to kinetic and biochemical analysis, and crystallized. The native enzyme is a homodimer with a subunit molecular mass of 38 kDa. The saturation curve for OMP as a substrate conformed to Michaelis-Menten kinetics with K m = 350 +/- 60 nM and V max = 2.70 +/- 0.10 micromol/min/mg protein. Inhibition patterns for nucleoside 5'-monophosphate analogues were linear competitive with respect to OMP with a decreasing potency of inhibition of PfODCase in the order: pyrazofurin 5'-monophosphate ( K i = 3.6 +/- 0.7 nM) &gt; xanthosine 5'-monophosphate (XMP, K i = 4.4 +/- 0.7 nM) &gt; 6-azauridine 5'-monophosphate (AzaUMP, K i = 12 +/- 3 nM) &gt; allopurinol-3-riboside 5'-monophosphate ( K i = 240 +/- 20 nM). XMP is an approximately 150-fold more potent inhibitor of PfODCase compared with the human enzyme. The structure of PfODCase was solved in the absence of ligand and displays a classic TIM-barrel fold characteristic of the enzyme. Both the phosphate-binding loop and the betaalpha5-loop have conformational flexibility, which may be associated with substrate capture and product release along the reaction pathway.
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==About this Structure==
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Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum.,Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI Biochemistry. 2008 Feb 28;. PMID:18303855<ref>PMID:18303855</ref>
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2ZCG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum., Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI, Biochemistry. 2008 Feb 28;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18303855 18303855]
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</div>
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[[Category: Orotidine-5'-phosphate decarboxylase]]
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<div class="pdbe-citations 2zcg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Single protein]]
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[[Category: Christopherson RI]]
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[[Category: Christopherson, R I.]]
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[[Category: Guss JM]]
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[[Category: Guss, J M.]]
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[[Category: Langley DB]]
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[[Category: Langley, D B.]]
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[[Category: Shojaei M]]
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[[Category: Shojaei, M.]]
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[[Category: Decarboxylase]]
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[[Category: Lyase]]
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[[Category: Pyrimidine biosynthesis]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:08:40 2008''
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Current revision

Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum

PDB ID 2zcg

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