This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3aaz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:17, 1 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3aaz.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of the humanized recombinant Fab fragment of a murine; antibody==
-
The line below this paragraph, containing "STRUCTURE_3aaz", creates the "Structure Box" on the page.
+
<StructureSection load='3aaz' size='340' side='right'caption='[[3aaz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3aaz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AAZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aaz OCA], [https://pdbe.org/3aaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aaz RCSB], [https://www.ebi.ac.uk/pdbsum/3aaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aaz ProSAT]</span></td></tr>
-
{{STRUCTURE_3aaz| PDB=3aaz | SCENE= }}
+
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/3aaz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3aaz ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Alzheimer's disease is the most common form of dementia, affecting 26 million people worldwide. The Abeta peptide (39-43 amino acids) derived from the proteolytic cleavage of the amyloid precursor protein is one of the main constituents of amyloid plaques associated with disease pathogenesis and therefore a validated target for therapy. Recently, we characterized antibody fragments (Fab and scFvs) derived from the murine monoclonal antibody WO-2, which bind the immunodominant epitope ((3)EFRH(6)) in the Abeta peptide at the N-terminus. In vitro, these fragments are able to inhibit fibril formation, disaggregate preformed amyloid fibrils, and protect neuroblastoma cells against oligomer-mediated toxicity. In this study, we describe the humanization of WO-2 using complementary determining region loop grafting onto the human germline gene and the determination of the three-dimensional structure by X-ray crystallography. This humanized version retains a high affinity for the Abeta peptide and therefore is a potential candidate for passive immunotherapy of Alzheimer's disease.
-
===Crystal structure of the humanized recombinant Fab fragment of a murine; antibody===
+
Germline humanization of a murine Abeta antibody and crystal structure of the humanized recombinant Fab fragment.,Robert R, Streltsov VA, Newman J, Pearce LA, Wark KL, Dolezal O Protein Sci. 2010 Feb;19(2):299-308. PMID:20014445<ref>PMID:20014445</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3aaz" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_20014445}}, adds the Publication Abstract to the page
+
*[[Antibody 3D structures|Antibody 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 20014445 is the PubMed ID number.
+
*[[Sandbox 20009|Sandbox 20009]]
-
-->
+
*[[3D structures of human antibody|3D structures of human antibody]]
-
{{ABSTRACT_PUBMED_20014445}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
3AAZ is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAZ OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:20014445</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Streltsov, V A.]]
+
[[Category: Large Structures]]
-
[[Category: Antibody engineering]]
+
[[Category: Streltsov VA]]
-
[[Category: Humanization]]
+
-
[[Category: Hwo-2 fab]]
+
-
[[Category: Immune system]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 16:00:07 2010''
+

Current revision

Crystal structure of the humanized recombinant Fab fragment of a murine; antibody

PDB ID 3aaz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3aaz"
Personal tools