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3b9r

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[[Image:3b9r.jpg|left|200px]]
 
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{{Structure
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==SERCA Ca2+-ATPase E2 aluminium fluoride complex without thapsigargin==
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|PDB= 3b9r |SIZE=350|CAPTION= <scene name='initialview01'>3b9r</scene>, resolution 3.000&Aring;
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<StructureSection load='3b9r' size='340' side='right'caption='[[3b9r]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER'>ACP</scene>
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<table><tr><td colspan='2'>[[3b9r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B9R FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b9r OCA], [https://pdbe.org/3b9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b9r RCSB], [https://www.ebi.ac.uk/pdbsum/3b9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b9r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/3b9r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b9r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The sarcoplasmic reticulum Ca2+-ATPase, a P-type ATPase, has a critical role in muscle function and metabolism. Here we present functional studies and three new crystal structures of the rabbit skeletal muscle Ca2+-ATPase, representing the phosphoenzyme intermediates associated with Ca2+ binding, Ca2+ translocation and dephosphorylation, that are based on complexes with a functional ATP analogue, beryllium fluoride and aluminium fluoride, respectively. The structures complete the cycle of nucleotide binding and cation transport of Ca2+-ATPase. Phosphorylation of the enzyme triggers the onset of a conformational change that leads to the opening of a luminal exit pathway defined by the transmembrane segments M1 through M6, which represent the canonical membrane domain of P-type pumps. Ca2+ release is promoted by translocation of the M4 helix, exposing Glu 309, Glu 771 and Asn 796 to the lumen. The mechanism explains how P-type ATPases are able to form the steep electrochemical gradients required for key functions in eukaryotic cells.
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'''SERCA Ca2+-ATPase E2 aluminium fluoride complex without thapsigargin'''
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The structural basis of calcium transport by the calcium pump.,Olesen C, Picard M, Winther AM, Gyrup C, Morth JP, Oxvig C, Moller JV, Nissen P Nature. 2007 Dec 13;450(7172):1036-42. PMID:18075584<ref>PMID:18075584</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3b9r" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3B9R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9R OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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[[Category: Calcium-transporting ATPase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Moller JV]]
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[[Category: Moller, J V.]]
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[[Category: Morth JP]]
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[[Category: Morth, J P.]]
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[[Category: Nissen P]]
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[[Category: Nissen, P.]]
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[[Category: Olesen C]]
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[[Category: Olesen, C.]]
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[[Category: Picard M]]
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[[Category: Picard, M.]]
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[[Category: Winther AML]]
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[[Category: Winther, A M.L.]]
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[[Category: ACP]]
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[[Category: ALF]]
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[[Category: K]]
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[[Category: MG]]
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[[Category: alternative splicing]]
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[[Category: aluminium fluoride]]
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[[Category: atp-binding]]
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[[Category: calcium]]
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[[Category: calcium pump]]
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[[Category: calcium transport]]
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[[Category: dephosphorylation]]
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[[Category: endoplasmic reticulum]]
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[[Category: hydrolase]]
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[[Category: ion transport]]
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[[Category: magnesium]]
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[[Category: membrane protein]]
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[[Category: metal-binding]]
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[[Category: nucleotide-binding]]
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[[Category: phosphorylation]]
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[[Category: sarcoplasmic reticulum]]
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[[Category: transition state]]
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[[Category: transmembrane]]
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[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:57:31 2008''
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Current revision

SERCA Ca2+-ATPase E2 aluminium fluoride complex without thapsigargin

PDB ID 3b9r

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