This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3bfd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:45, 1 November 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3bfd.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of the Class A beta-lactamase SED-G238C mutant from Citrobacter sedlakii==
-
The line below this paragraph, containing "STRUCTURE_3bfd", creates the "Structure Box" on the page.
+
<StructureSection load='3bfd' size='340' side='right'caption='[[3bfd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3bfd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BFD FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene></td></tr>
-
{{STRUCTURE_3bfd| PDB=3bfd | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bfd OCA], [https://pdbe.org/3bfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bfd RCSB], [https://www.ebi.ac.uk/pdbsum/3bfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bfd ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q93PQ0_9ENTR Q93PQ0_9ENTR]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/3bfd_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bfd ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of the Class A beta-lactamase SED-G238C mutant from Citrobacter sedlakii'''
+
==See Also==
-
 
+
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C.
+
-
 
+
-
==About this Structure==
+
-
3BFD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFD OCA].
+
-
 
+
-
==Reference==
+
-
Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684905 14684905]
+
-
[[Category: Beta-lactamase]]
+
[[Category: Citrobacter sedlakii]]
[[Category: Citrobacter sedlakii]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Pernot, L.]]
+
[[Category: Pernot L]]
-
[[Category: Petrella, S.]]
+
[[Category: Petrella S]]
-
[[Category: Sougakoff, W.]]
+
[[Category: Sougakoff W]]
-
[[Category: Beta-lactamase]]
+
-
[[Category: Class some]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Sed-g238c mutant]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:43:00 2008''
+

Current revision

Crystal Structure of the Class A beta-lactamase SED-G238C mutant from Citrobacter sedlakii

PDB ID 3bfd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bfd"
Personal tools