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| | ==Crystal structure of human BTG2== | | ==Crystal structure of human BTG2== |
| - | <StructureSection load='3dju' size='340' side='right' caption='[[3dju]], [[Resolution|resolution]] 2.26Å' scene=''> | + | <StructureSection load='3dju' size='340' side='right'caption='[[3dju]], [[Resolution|resolution]] 2.26Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3dju]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DJU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3dju]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DJU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3djn|3djn]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BTG2, B-cell translocation gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dju OCA], [https://pdbe.org/3dju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dju RCSB], [https://www.ebi.ac.uk/pdbsum/3dju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dju ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dju OCA], [http://pdbe.org/3dju PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dju RCSB], [http://www.ebi.ac.uk/pdbsum/3dju PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dju ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BTG2_HUMAN BTG2_HUMAN]] Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Anti-proliferative protein. Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.<ref>PMID:12771185</ref> <ref>PMID:15788397</ref> <ref>PMID:18773938</ref> | + | [https://www.uniprot.org/uniprot/BTG2_HUMAN BTG2_HUMAN] Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Anti-proliferative protein. Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.<ref>PMID:12771185</ref> <ref>PMID:15788397</ref> <ref>PMID:18773938</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Bartlam, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Morita, M]] | + | [[Category: Bartlam M]] |
| - | [[Category: Suzuki, T]] | + | [[Category: Morita M]] |
| - | [[Category: Wang, H]] | + | [[Category: Suzuki T]] |
| - | [[Category: Yamamoto, T]] | + | [[Category: Wang H]] |
| - | [[Category: Yang, X]] | + | [[Category: Yamamoto T]] |
| - | [[Category: Helix-turn-helix]]
| + | [[Category: Yang X]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Function
BTG2_HUMAN Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Anti-proliferative protein. Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
BTG2 is the prototypical member of the TOB family and is known to be involved in cell growth, differentiation and DNA repair. As a transcriptional co-regulator, BTG2 interacts with CCR4-associated factor 1 (CAF1) and POP2 (CALIF), which are key components of the general CCR4/NOT multi-subunit transcription complex, and which are reported to play distinct roles as nucleases involved in mRNA deadenylation. Here we report the crystal structures of human BTG2 and mouse TIS21 to 2.3 A and 2.2 A resolution, respectively. The structures reveal the putative CAF1 binding site. CAF1 deadenylase assays were performed with wild-type BTG2 and mutants that disrupt the interaction with CAF1. The results reveal the suppressive role of BTG2 in the regulation of CAF1 deadenylase activity. Our study provides insights into the formation of the BTG2-CAF1 complex and the potential role of BTG2 in the regulation of CAF1.
Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity.,Yang X, Morita M, Wang H, Suzuki T, Yang W, Luo Y, Zhao C, Yu Y, Bartlam M, Yamamoto T, Rao Z Nucleic Acids Res. 2008 Dec;36(21):6872-81. Epub 2008 Oct 30. PMID:18974182[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morel AP, Sentis S, Bianchin C, Le Romancer M, Jonard L, Rostan MC, Rimokh R, Corbo L. BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms. J Cell Sci. 2003 Jul 15;116(Pt 14):2929-36. Epub 2003 May 27. PMID:12771185 doi:http://dx.doi.org/10.1242/jcs.00480
- ↑ Hong JW, Ryu MS, Lim IK. Phosphorylation of serine 147 of tis21/BTG2/pc3 by p-Erk1/2 induces Pin-1 binding in cytoplasm and cell death. J Biol Chem. 2005 Jun 3;280(22):21256-63. Epub 2005 Mar 23. PMID:15788397 doi:10.1074/jbc.M500318200
- ↑ Miyata S, Mori Y, Tohyama M. PRMT1 and Btg2 regulates neurite outgrowth of Neuro2a cells. Neurosci Lett. 2008 Nov 14;445(2):162-5. doi: 10.1016/j.neulet.2008.08.065. Epub , 2008 Aug 28. PMID:18773938 doi:http://dx.doi.org/10.1016/j.neulet.2008.08.065
- ↑ Yang X, Morita M, Wang H, Suzuki T, Yang W, Luo Y, Zhao C, Yu Y, Bartlam M, Yamamoto T, Rao Z. Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity. Nucleic Acids Res. 2008 Dec;36(21):6872-81. Epub 2008 Oct 30. PMID:18974182 doi:10.1093/nar/gkn825
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