3e6e

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{{Seed}}
 
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[[Image:3e6e.png|left|200px]]
 
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==Crystal structure of Alanine racemase from E.faecalis complex with cycloserine==
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The line below this paragraph, containing "STRUCTURE_3e6e", creates the "Structure Box" on the page.
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<StructureSection load='3e6e' size='340' side='right'caption='[[3e6e]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e6e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E6E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCS:D-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-N,O-CYCLOSERYLAMIDE'>DCS</scene></td></tr>
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{{STRUCTURE_3e6e| PDB=3e6e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e6e OCA], [https://pdbe.org/3e6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e6e RCSB], [https://www.ebi.ac.uk/pdbsum/3e6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e6e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q837J0_ENTFA Q837J0_ENTFA] Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.[HAMAP-Rule:MF_01201]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e6/3e6e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e6e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Alanine racemase (AlaR) is a bacterial enzyme that belongs to the fold-type III group of pyridoxal 5'-phosphate (PLP)-dependent enzymes. AlaR catalyzes the interconversion between L- and D-alanine, which is important for peptidoglycan biosynthesis. This enzyme is common in prokaryotes, but absent in eukaryotes, which makes it an attractive target for the design of new antibacterial drugs. Here, we report the crystal structures of both the apoenzyme and the d-cycloserine (DCS) complex of AlaR from the pathogenic bacterium Enterococcus faecalis v583, at a resolution of 2.5 A. DCS is a suicide inhibitor of AlaR and, as such, serves as an antimicrobial agent and has been used to treat tuberculosis and urinary tract infection-related diseases, and makes several hydrogen bonds with the conserved active site residues, Tyr44 and Ser207, respectively. The apoenzyme crystal structure of AlaR consists of three monomers in the asymmetric unit, including a polyethylene glycol molecule in the dimer interface that surrounds one of the His 293 residues and also sits close to one side of the His 293 residue in the opposite monomer. Our results provide structural insights into AlaR that may be used for the development of new antibiotics targeting the alanine racemase in pathogenic bacteria.
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===Crystal structure of Alanine racemase from E.faecalis complex with cycloserine===
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Structural insights into the alanine racemase from Enterococcus faecalis.,Priyadarshi A, Lee EH, Sung MW, Nam KH, Lee WH, Kim EE, Hwang KY Biochim Biophys Acta. 2009 Jul;1794(7):1030-40. Epub 2009 Mar 26. PMID:19328247<ref>PMID:19328247</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3e6e" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19328247}}, adds the Publication Abstract to the page
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*[[Alanine racemase 3D structures|Alanine racemase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19328247 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19328247}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3E6E is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E6E OCA].
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==Reference==
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<ref group="xtra">PMID:19328247</ref><references group="xtra"/>
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[[Category: Alanine racemase]]
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[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
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[[Category: Hwang, K Y.]]
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[[Category: Large Structures]]
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[[Category: Lee, E H.]]
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[[Category: Hwang KY]]
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[[Category: Priyadarshi, A.]]
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[[Category: Lee EH]]
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[[Category: Sung, M W.]]
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[[Category: Priyadarshi A]]
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[[Category: Alr]]
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[[Category: Sung MW]]
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[[Category: Isomerase]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Scp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:03:11 2009''
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Current revision

Crystal structure of Alanine racemase from E.faecalis complex with cycloserine

PDB ID 3e6e

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