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3fpx
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==Native fungus laccase from Trametes hirsuta== | ==Native fungus laccase from Trametes hirsuta== | ||
| - | <StructureSection load='3fpx' size='340' side='right' caption='[[3fpx]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3fpx' size='340' side='right'caption='[[3fpx]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3fpx]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3fpx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trametes_hirsuta Trametes hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpx OCA], [https://pdbe.org/3fpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpx RCSB], [https://www.ebi.ac.uk/pdbsum/3fpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpx ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B2L9C1_TRAHI B2L9C1_TRAHI] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpx ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpx ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map. | ||
| - | + | ==See Also== | |
| - | + | *[[Laccase 3D structures|Laccase 3D structures]] | |
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| - | == | + | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
[[Category: Trametes hirsuta]] | [[Category: Trametes hirsuta]] | ||
| - | [[Category: Cherkashin | + | [[Category: Cherkashin EA]] |
| - | [[Category: Fedorova | + | [[Category: Fedorova TV]] |
| - | [[Category: Koroleva | + | [[Category: Koroleva OV]] |
| - | [[Category: Kurzeev | + | [[Category: Kurzeev SA]] |
| - | [[Category: Lamzin | + | [[Category: Lamzin VS]] |
| - | [[Category: Polyakov | + | [[Category: Polyakov KM]] |
| - | [[Category: Stepanova | + | [[Category: Stepanova EV]] |
| - | [[Category: Strokopytov | + | [[Category: Strokopytov BV]] |
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Current revision
Native fungus laccase from Trametes hirsuta
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