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| | ==Crystal Structure of the hypothetical egulator from Sulfolobus tokodaii 7== | | ==Crystal Structure of the hypothetical egulator from Sulfolobus tokodaii 7== |
| - | <StructureSection load='3gez' size='340' side='right' caption='[[3gez]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3gez' size='340' side='right'caption='[[3gez]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3gez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GEZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3gez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GEZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2eb7|2eb7]], [[2yr2|2yr2]], [[3gf2|3gf2]], [[3gfi|3gfi]], [[3gfj|3gfj]], [[3gfl|3gfl]], [[3gfm|3gfm]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST1710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gez OCA], [https://pdbe.org/3gez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gez RCSB], [https://www.ebi.ac.uk/pdbsum/3gez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gez ProSAT], [https://www.topsan.org/Proteins/RSGI/3gez TOPSAN]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gez OCA], [http://pdbe.org/3gez PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gez RCSB], [http://www.ebi.ac.uk/pdbsum/3gez PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gez ProSAT], [http://www.topsan.org/Proteins/RSGI/3gez TOPSAN]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q96ZY1_SULTO Q96ZY1_SULTO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dsm 16993]] | + | [[Category: Large Structures]] |
| - | [[Category: Kumarevel, T]] | + | [[Category: Sulfurisphaera tokodaii]] |
| - | [[Category: Structural genomic]] | + | [[Category: Kumarevel T]] |
| - | [[Category: Tanaka, T]] | + | [[Category: Tanaka T]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Yokoyama S]] |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Marr]]
| + | |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Regulator]]
| + | |
| - | [[Category: Rsgi]]
| + | |
| - | [[Category: St1710]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
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| Structural highlights
Function
Q96ZY1_SULTO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism.
ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.,Kumarevel T, Tanaka T, Umehara T, Yokoyama S Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumarevel T, Tanaka T, Umehara T, Yokoyama S. ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators. Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310 doi:10.1093/nar/gkp496
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