This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3i4c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:55, 1 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3i4c.png|left|200px]]
 
-
<!--
+
==Crystal structure of Sulfolobus Solfataricus ADH(SsADH) double mutant (W95L,N249Y)==
-
The line below this paragraph, containing "STRUCTURE_3i4c", creates the "Structure Box" on the page.
+
<StructureSection load='3i4c' size='340' side='right'caption='[[3i4c]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3i4c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I4C FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_3i4c| PDB=3i4c | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i4c OCA], [https://pdbe.org/3i4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i4c RCSB], [https://www.ebi.ac.uk/pdbsum/3i4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i4c ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ADH_SACS2 ADH_SACS2]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/3i4c_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i4c ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A mutant of the thermostable NAD(+)-dependent (S)-stereospecific alcohol dehydrogenase from Sulfolobus solfataricus (SsADH) which has a single substitution, Trp95Leu, located at the substrate binding pocket, was fully characterized to ascertain the role of Trp95 in discriminating between chiral secondary alcohols suggested by the wild-type SsADH crystallographic structure. The Trp95Leu mutant displays no apparent activity with short-chain primary and secondary alcohols and poor activity with aromatic substrates and coenzyme. Moreover, the Trp --&gt; Leu substitution affects the structural stability of the archaeal ADH, decreasing its thermal stability without relevant changes in secondary structure. The double mutant Trp95Leu/Asn249Tyr was also purified to assist in crystallographic analysis. This mutant exhibits higher activity but decreased affinity toward aliphatic alcohols, aldehydes as well as NAD(+) and NADH compared to the wild-type enzyme. The crystal structure of the Trp95Leu/Asn249Tyr mutant apo form, determined at 2.0 A resolution, reveals a large local rearrangement of the substrate site with dramatic consequences. The Leu95 side-chain conformation points away from the catalytic metal center and the widening of the substrate site is partially counteracted by a concomitant change of Trp117 side chain conformation. Structural changes at the active site are consistent with the reduced activity on substrates and decreased coenzyme binding.
-
===Crystal structure of Sulfolobus Solfataricus ADH(SsADH) double mutant (W95L,N249Y)===
+
Role of Tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase.,Pennacchio A, Esposito L, Zagari A, Rossi M, Raia CA Extremophiles. 2009 Jul 9. PMID:19588068<ref>PMID:19588068</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3i4c" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19588068}}, adds the Publication Abstract to the page
+
*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19588068 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19588068}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
3I4C is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I4C OCA].
+
[[Category: Saccharolobus solfataricus]]
-
 
+
[[Category: Esposito L]]
-
==Reference==
+
[[Category: Pennacchio A]]
-
<ref group="xtra">PMID:19588068</ref><references group="xtra"/>
+
[[Category: Raia CA]]
-
[[Category: Alcohol dehydrogenase]]
+
[[Category: Rossi M]]
-
[[Category: Sulfolobus solfataricus]]
+
[[Category: Zagari A]]
-
[[Category: Esposito, L.]]
+
-
[[Category: Pennacchio, A.]]
+
-
[[Category: Raia, C A.]]
+
-
[[Category: Rossi, M.]]
+
-
[[Category: Zagari, A.]]
+
-
[[Category: Archaeon]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Methylation]]
+
-
[[Category: Mutant]]
+
-
[[Category: Nad]]
+
-
[[Category: Oxidoreductase]]
+
-
[[Category: Zinc]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:25:59 2009''
+

Current revision

Crystal structure of Sulfolobus Solfataricus ADH(SsADH) double mutant (W95L,N249Y)

PDB ID 3i4c

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3i4c"
Personal tools