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| | ==Crystal structure of Cren7-dsDNA complex== | | ==Crystal structure of Cren7-dsDNA complex== |
| - | <StructureSection load='3lwh' size='340' side='right' caption='[[3lwh]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3lwh' size='340' side='right'caption='[[3lwh]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3lwh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LWH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3lwh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LWH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lwi|3lwi]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">creN7, SSO6901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lwh OCA], [https://pdbe.org/3lwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lwh RCSB], [https://www.ebi.ac.uk/pdbsum/3lwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lwh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lwh OCA], [http://pdbe.org/3lwh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lwh RCSB], [http://www.ebi.ac.uk/pdbsum/3lwh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lwh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CREN7_SULSO CREN7_SULSO]] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref> | + | [https://www.uniprot.org/uniprot/CREN7_SACS2 CREN7_SACS2] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sacs2]] | + | [[Category: Large Structures]] |
| - | [[Category: Gong, Y]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Guo, L]] | + | [[Category: Gong Y]] |
| - | [[Category: Huang, L]] | + | [[Category: Guo L]] |
| - | [[Category: Jiang, T]] | + | [[Category: Huang L]] |
| - | [[Category: Zhang, Z F]] | + | [[Category: Jiang T]] |
| - | [[Category: Beta-sheet]]
| + | [[Category: Zhang ZF]] |
| - | [[Category: Dna binding protein-dna complex]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Methylation]]
| + | |
| - | [[Category: Protein-dna complex]]
| + | |
| Structural highlights
Function
CREN7_SACS2 A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cren7, a newly found chromatin protein, is highly conserved in the Crenarchaeota. The protein shows higher affinity for double-stranded DNA than for single-stranded DNA, constrains negative DNA supercoils in vitro and is associated with genomic DNA in vivo. Here we report the crystal structures of the Cren7 protein from Sulfolobus solfataricus in complex with two DNA sequences. Cren7 binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 53 degrees) through the intercalation of the hydrophobic side chain of Leu28. Loop beta 3-beta 4 of Cren7 undergoes a significant conformational change upon binding of the protein to DNA, suggesting its critical role in the stabilization of the protein-DNA complex. The roles of DNA-contacting amino acid residues in stabilizing the Cren7-DNA interaction were examined by mutational analysis. Structural comparison of Cren7-DNA complexes with Sac7d-DNA complexes reveals significant differences between the two proteins in DNA binding surface, suggesting that Cren7 and Sul7d serve distinct functions in chromosomal organization.
Structural insights into the interaction of the crenarchaeal chromatin protein Cren7 with DNA.,Zhang Z, Gong Y, Guo L, Jiang T, Huang L Mol Microbiol. 2010 May;76(3):749-59. Epub 2010 Mar 16. PMID:20345658[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guo L, Feng Y, Zhang Z, Yao H, Luo Y, Wang J, Huang L. Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 2008 Mar;36(4):1129-37. Epub 2007 Dec 20. PMID:18096617 doi:10.1093/nar/gkm1128
- ↑ Zhang Z, Gong Y, Guo L, Jiang T, Huang L. Structural insights into the interaction of the crenarchaeal chromatin protein Cren7 with DNA. Mol Microbiol. 2010 May;76(3):749-59. Epub 2010 Mar 16. PMID:20345658 doi:10.1111/j.1365-2958.2010.07136.x
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