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3ml0

From Proteopedia

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[[Image:3ml0.png|left|200px]]
 
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==Thermostable Penicillin G acylase from Alcaligenes faecalis in tetragonal form==
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The line below this paragraph, containing "STRUCTURE_3ml0", creates the "Structure Box" on the page.
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<StructureSection load='3ml0' size='340' side='right'caption='[[3ml0]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ml0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ML0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_3ml0| PDB=3ml0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ml0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ml0 OCA], [https://pdbe.org/3ml0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ml0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ml0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ml0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O34142_ALCFA O34142_ALCFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/3ml0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ml0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme penicillin G acylase (EC 3.5.1.11) catalyzes amide-bond cleavage in benzylpenicillin (penicillin G) to yield 6-aminopenicillanic acid, an intermediate chemical used in the production of semisynthetic penicillins. A thermostable penicillin G acylase from Alcaligenes faecalis (AfPGA) has been crystallized using the hanging-drop vapour-diffusion method in two different space groups: C222(1), with unit-cell parameters a = 72.9, b = 86.0, c = 260.2 , and P4(1)2(1)2, with unit-cell parameters a = b = 85.6, c = 298.8 . Data were collected at 293 and the structure was determined using the molecular-replacement method. Like other penicillin acylases, AfPGA belongs to the N-terminal nucleophilic hydrolase superfamily, has undergone post-translational processing and has a serine as the N-terminal residue of the beta-chain. A disulfide bridge has been identified in the structure that was not found in the other two known penicillin G cylase structures. The presence of the disulfide bridge is perceived to be one factor that confers higher stability to this enzyme.
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===Thermostable Penicillin G acylase from Alcaligenes faecalis in tetragonal form===
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Crystallization and X-ray structure analysis of a thermostable penicillin G acylase from Alcaligenes faecalis.,Varshney NK, Kumar RS, Ignatova Z, Prabhune A, Pundle A, Dodson E, Suresh CG Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):273-7. Epub, 2012 Feb 15. PMID:22442220<ref>PMID:22442220</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ml0" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3ml0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML0 OCA].
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*[[Penicillin acylase|Penicillin acylase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
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[[Category: Penicillin amidase]]
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[[Category: Large Structures]]
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[[Category: Dodson, E.]]
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[[Category: Dodson E]]
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[[Category: Ignatova, Z.]]
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[[Category: Ignatova Z]]
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[[Category: Kumar, R S.]]
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[[Category: Kumar RS]]
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[[Category: Suresh, C G.]]
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[[Category: Suresh CG]]
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[[Category: Varshney, N K.]]
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[[Category: Varshney NK]]
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[[Category: Hydrolase]]
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[[Category: Penicillin g acylase]]
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Current revision

Thermostable Penicillin G acylase from Alcaligenes faecalis in tetragonal form

PDB ID 3ml0

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