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| | ==The structure of chi1, a chitinase from Yersinia entomophaga== | | ==The structure of chi1, a chitinase from Yersinia entomophaga== |
| - | <StructureSection load='3oa5' size='340' side='right' caption='[[3oa5]], [[Resolution|resolution]] 1.74Å' scene=''> | + | <StructureSection load='3oa5' size='340' side='right'caption='[[3oa5]], [[Resolution|resolution]] 1.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3oa5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_sp._mh-1 Yersinia sp. mh-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OA5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3oa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_entomophaga Yersinia entomophaga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OA5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chi1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373547 Yersinia sp. MH-1])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oa5 OCA], [https://pdbe.org/3oa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oa5 RCSB], [https://www.ebi.ac.uk/pdbsum/3oa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oa5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oa5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oa5 RCSB], [http://www.ebi.ac.uk/pdbsum/3oa5 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CHI1_YERET CHI1_YERET] Part of an orally active toxin complex (TC) with strong insecticidal effects on larvae of the Coleoptera Costelytra zealandica, Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera larvae (PubMed:21278295). The TC has an endochitinase activity (PubMed:21278295, PubMed:22158901) (Probable). This subunit might aid infection by degradation of the larval peritrophic membrane (Probable).<ref>PMID:21278295</ref> <ref>PMID:22158901</ref> <ref>PMID:22108167</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3oa5" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Chitinase|Chitinase]] | + | *[[Chitinase 3D structures|Chitinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chitinase]] | + | [[Category: Large Structures]] |
| - | [[Category: Yersinia sp. mh-1]] | + | [[Category: Yersinia entomophaga]] |
| - | [[Category: Busby, J N]] | + | [[Category: Busby JN]] |
| - | [[Category: Hurst, M R.H]] | + | [[Category: Hurst MRH]] |
| - | [[Category: Lott, J S]] | + | [[Category: Lott JS]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Tim barrel]]
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| Structural highlights
Function
CHI1_YERET Part of an orally active toxin complex (TC) with strong insecticidal effects on larvae of the Coleoptera Costelytra zealandica, Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera larvae (PubMed:21278295). The TC has an endochitinase activity (PubMed:21278295, PubMed:22158901) (Probable). This subunit might aid infection by degradation of the larval peritrophic membrane (Probable).[1] [2] [3]
Publication Abstract from PubMed
Yersinia entomophaga MH96 is a native New Zealand soil bacterium that secretes a large ABC-type protein toxin complex, Yen-Tc, similar to those produced by nematode-associated bacteria such as Photorhabdus luminescens. Y. entomophaga displays an exceptionally virulent pathogenic phenotype in sensitive insect species, causing death within 72 h of infection. Because of this phenotype, there is intrinsic interest in the mechanism of action of Yen-Tc, and it also has the potential to function as a novel class of biopesticides. We have identified genes that encode chitinases as part of the toxin complex loci in Y. entomophaga MH96, P. luminescens, Photorhabdus asymbiotica and Xenorhabdus nematophila. Furthermore, we have shown that the secreted toxin complex from Y. entomophaga MH96 includes two chitinases as an integral part of the complex, a feature not described previously in other ABC toxins and possibly related to the severe disease caused by this bacterium. We present here the structure of the Y. entomophaga MH96 Chi1 chitinase, determined by X-ray crystallography to 1.74 A resolution, and show that a ring of five symmetrically arranged lobes on the surface of the Yen-Tc toxin complex structure, as determined by single-particle electron microscopy, provides a good fit to the Chi1 monomer. We also confirm that the isolated chitinases display endochitinase activity, as does the complete toxin complex.
Structural Analysis of Chi1 Chitinase from Yen-Tc: The Multisubunit Insecticidal ABC Toxin Complex of Yersinia entomophaga.,Busby JN, Landsberg MJ, Simpson R, Jones SA, Hankamer B, Hurst MR, Lott JS J Mol Biol. 2011 Nov 15. PMID:22108167[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hurst MR, Jones SA, Binglin T, Harper LA, Jackson TA, Glare TR. The main virulence determinant of Yersinia entomophaga MH96 is a broad-host-range toxin complex active against insects. J Bacteriol. 2011 Apr;193(8):1966-80. doi: 10.1128/JB.01044-10. Epub 2011 Jan 28. PMID:21278295 doi:http://dx.doi.org/10.1128/JB.01044-10
- ↑ Landsberg MJ, Jones SA, Rothnagel R, Busby JN, Marshall SD, Simpson RM, Lott JS, Hankamer B, Hurst MR. 3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20544-9. doi:, 10.1073/pnas.1111155108. Epub 2011 Dec 7. PMID:22158901 doi:http://dx.doi.org/10.1073/pnas.1111155108
- ↑ Busby JN, Landsberg MJ, Simpson R, Jones SA, Hankamer B, Hurst MR, Lott JS. Structural Analysis of Chi1 Chitinase from Yen-Tc: The Multisubunit Insecticidal ABC Toxin Complex of Yersinia entomophaga. J Mol Biol. 2011 Nov 15. PMID:22108167 doi:10.1016/j.jmb.2011.11.018
- ↑ Busby JN, Landsberg MJ, Simpson R, Jones SA, Hankamer B, Hurst MR, Lott JS. Structural Analysis of Chi1 Chitinase from Yen-Tc: The Multisubunit Insecticidal ABC Toxin Complex of Yersinia entomophaga. J Mol Biol. 2011 Nov 15. PMID:22108167 doi:10.1016/j.jmb.2011.11.018
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