This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3og2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (16:55, 1 November 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3og2.png|left|200px]]
 
-
{{STRUCTURE_3og2| PDB=3og2 | SCENE= }}
+
==Native crystal structure of Trichoderma reesei beta-galactosidase==
 +
<StructureSection load='3og2' size='340' side='right'caption='[[3og2]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3og2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OG2 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3og2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3og2 OCA], [https://pdbe.org/3og2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3og2 RCSB], [https://www.ebi.ac.uk/pdbsum/3og2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3og2 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q70SY0_HYPJE Q70SY0_HYPJE]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We have determined the crystal structure of Trichoderma reesei (Hypocrea jecorina) beta-galactosidase (Tr-beta-gal) at a 1.2A resolution and its complex structures with galactose, IPTG and PETG at 1.5, 1.75 and 1.4A resolutions, respectively. Tr-beta-gal is a potential enzyme for lactose hydrolysis in the dairy industry and belongs to family 35 of the glycoside hydrolases (GH-35). The high resolution crystal structures of this six-domain enzyme revealed interesting features about the structure of Tr-beta-gal. We discovered conformational changes in the two loop regions in the active site, implicating a conformational selection-mechanism for the enzyme. In addition, the Glu200, an acid/base catalyst showed two different conformations which undoubtedly affect the pK(a) value of this residue and the catalytic mechanism. The electron density showed extensive glycosylation, suggesting a structure stabilizing role for glycans. The longest glycan showed an electron density that extends to the eighth monosaccharide unit in the extended chain. The Tr-beta-gal structure also showed a well-ordered structure for a unique octaserine motif on the surface loop of the fifth domain.
-
===Native crystal structure of Trichoderma reesei beta-galactosidase===
+
Crystal structures of Trichoderma reesei beta-galactosidase reveal conformational changes in the active site.,Maksimainen M, Hakulinen N, Kallio JM, Timoharju T, Turunen O, Rouvinen J J Struct Biol. 2011 Apr;174(1):156-63. Epub 2010 Dec 3. PMID:21130883<ref>PMID:21130883</ref>
-
{{ABSTRACT_PUBMED_21130883}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 3og2" style="background-color:#fffaf0;"></div>
-
[[3og2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OG2 OCA].
+
==See Also==
==See Also==
-
*[[Galactosidase|Galactosidase]]
+
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:021130883</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Beta-galactosidase]]
+
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Trichoderma reesei]]
[[Category: Trichoderma reesei]]
-
[[Category: Maksimainen, M.]]
+
[[Category: Maksimainen M]]
-
[[Category: Rouvinen, J.]]
+
[[Category: Rouvinen J]]
-
[[Category: Family 35]]
+
-
[[Category: Glycoprotein]]
+
-
[[Category: Glycoside hydrolase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Tim barrel domain]]
+

Current revision

Native crystal structure of Trichoderma reesei beta-galactosidase

PDB ID 3og2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3og2"
Personal tools